Single Conducting Polymer Nanowire Chemiresistive Label-Free Immunosensor for Cancer Biomarker

被引:138
作者
Bangar, Mangesh A.
Shirale, Dhammanand J.
Chen, Wilfred
Myung, Nosang V.
Mulchandani, Ashok [1 ]
机构
[1] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA
关键词
TUMOR-ASSOCIATED ANTIGEN; MONOCLONAL-ANTIBODY; OVARIAN-CANCER; CA; 125; PROTEIN; SENSOR; FABRICATION; NANOTUBES; CA125; FILM;
D O I
10.1021/ac802319f
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple and cost-effective, all-electrochemical method to fabricate and assemble single conducting polymer nanowire based biosensors was developed. Polypyrrole (Ppy) nanowires were synthesized by electrochemical polymerization using an alumina template. The single-nanowire chemoresistive sensor device was assembled using ac dielectrophoretic alignment followed by maskless anchoring on a pair of gold electrodes separated by 3 mu m. To establish an efficient covalent surface biofunctionalization route, glutaraldehyde (GA) and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) chemistries were compared. EDC was established to be the most effective chemistry and was used to surface-functionalize a single Ppy nanowire with cancer antigen (CA 125) antibody to fabricate a nanoimmunosensor for CA 125 biomarker detection and quantification. The immunosensor had excellent sensitivity with a lower detection limit of 1 U/mL CA 125 and dynamic range up to 1000 U/mL in 10 mM phosphate buffer. Furthermore, there was no loss of performance upon exposure to CA 125 in spiked human blood plasma. This demonstrates the clinical importance of these sensors for cancer marker detection with cost benefits and great portability for diagnosis of patients at the point of care.
引用
收藏
页码:2168 / 2175
页数:8
相关论文
共 47 条
[1]   Proposed reference value of the CA 125 tumour marker in men.: Potential applications in clinical practice [J].
Barcelo, B. ;
Ayllon, O. ;
Belmonte, M. ;
Barcelo, A. ;
Vidal, R. ;
Forteza-Rey, J. ;
Gutierrez, A. .
CLINICAL BIOCHEMISTRY, 2008, 41 (09) :717-722
[2]   A RADIOIMMUNOASSAY USING A MONOCLONAL-ANTIBODY TO MONITOR THE COURSE OF EPITHELIAL OVARIAN-CANCER [J].
BAST, RC ;
KLUG, TL ;
STJOHN, E ;
JENISON, E ;
NILOFF, JM ;
LAZARUS, H ;
BERKOWITZ, RS ;
LEAVITT, T ;
GRIFFITHS, CT ;
PARKER, L ;
ZURAWSKI, VR ;
KNAPP, RC .
NEW ENGLAND JOURNAL OF MEDICINE, 1983, 309 (15) :883-887
[3]   REACTIVITY OF A MONOCLONAL-ANTIBODY WITH HUMAN OVARIAN-CARCINOMA [J].
BAST, RC ;
FEENEY, M ;
LAZARUS, H ;
NADLER, LM ;
COLVIN, RB ;
KNAPP, RC .
JOURNAL OF CLINICAL INVESTIGATION, 1981, 68 (05) :1331-1337
[4]   CA 125: The past and the future [J].
Bast, RC ;
Xu, FJ ;
Yu, YH ;
Barnhill, S ;
Zhang, Z ;
Mills, GB .
INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS, 1998, 13 (04) :179-187
[5]   OVARIAN-CANCER ANTIGEN CA125 - A PROSPECTIVE CLINICAL-ASSESSMENT OF ITS ROLE AS A TUMOR-MARKER [J].
CANNEY, PA ;
MOORE, M ;
WILKINSON, PM ;
JAMES, RD .
BRITISH JOURNAL OF CANCER, 1984, 50 (06) :765-769
[6]   Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors [J].
Chen, RJ ;
Bangsaruntip, S ;
Drouvalakis, KA ;
Kam, NWS ;
Shim, M ;
Li, YM ;
Kim, W ;
Utz, PJ ;
Dai, HJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (09) :4984-4989
[7]   Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species [J].
Cui, Y ;
Wei, QQ ;
Park, HK ;
Lieber, CM .
SCIENCE, 2001, 293 (5533) :1289-1292
[8]   Dielectrophoretically assembled polymer nanowires for gas sensing [J].
Dan, Yaping ;
Cao, Yanyan ;
Mallouk, Tom E. ;
Johnson, Alan T. ;
Evoy, Stephane .
SENSORS AND ACTUATORS B-CHEMICAL, 2007, 125 (01) :55-59
[9]   Cholesterol biosensor based on electrophoretically deposited conducting polymer film derived from nano-structured polyaniline colloidal suspension [J].
Dhand, Chetna ;
Singh, S. P. ;
Arya, Sunil K. ;
Datta, Blonika ;
Malhotra, B. D. .
ANALYTICA CHIMICA ACTA, 2007, 602 (02) :244-251
[10]  
DI-XIA C, 1988, Obstetrics and Gynecology, V72, P23