Quantitative detection of respiratory Chlamydia pneumoniae infection by real-time PCR

被引：66

作者：

Kuoppa, Y ^{[1
]}

Boman, J ^{[1
]}

Scott, L ^{[1
]}

Kumlin, U ^{[1
]}

Eriksson, I ^{[1
]}

Allard, A ^{[1
]}

机构：

[1] Umea Univ, Dept Virol, S-90185 Umea, Sweden

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 06期

关键词：

D O I：

10.1128/JCM.40.6.2273-2274.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Real-time PCR was evaluated as a quantitative diagnostic method for Chlamydia pneumoniae infection using different respiratory samples. Real-time PCR had efficiency equal to or better than that of nested touchdown PCR. This study confirmed sputum as the best sampling material to detect an ongoing C. pneumoniae infection.

引用

收藏

页码：2273 / 2274

页数：2

相关论文

共 11 条

[1] Molecular diagnosis of Chlamydia pneumoniae infection [J].

Boman, J ;

Gaydos, CA ;

Quinn, TC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (12) :3791-3799

[2] Rapid diagnosis of respiratory Chlamydia pneumoniae infection by nested touchdown polymerase chain reaction compared with culture and antigen detection by EIA [J].

Boman, J ;

Allard, A ;

Persson, K ;

Lundborg, M ;

Juto, P ;

Wadell, G .

JOURNAL OF INFECTIOUS DISEASES, 1997, 175 (06) :1523-1526

[3] Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis [J].

Desjardin, LE ;

Chen, Y ;

Perkins, MD ;

Teixeira, L ;

Cave, MD ;

Eisenach, KD .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) :1964-1968

[4] TOUCHDOWN PCR TO CIRCUMVENT SPURIOUS PRIMING DURING GENE AMPLIFICATION [J].

DON, RH ;

COX, PT ;

WAINWRIGHT, BJ ;

BAKER, K ;

MATTICK, JS .

NUCLEIC ACIDS RESEARCH, 1991, 19 (14) :4008-4008

[5] Quantification of human cytomegalovirus DNA by real-time PCR [J].

Gault, E ;

Michel, Y ;

Dehée, A ;

Belabani, C ;

Nicolas, JC ;

Garbarg-Chenon, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (02) :772-775

[6] A NEW RESPIRATORY-TRACT PATHOGEN - CHLAMYDIA-PNEUMONIAE STRAIN TWAR [J].

GRAYSTON, JT ;

CAMPBELL, LA ;

KUO, CC ;

MORDHORST, CH ;

SAIKKU, P ;

THOM, DH ;

WANG, SP .

JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (04) :618-625

[7] CHLAMYDIA-PNEUMONIAE (TWAR) [J].

KUO, CC ;

JACKSON, LA ;

CAMPBELL, LA ;

GRAYSTON, JT .

CLINICAL MICROBIOLOGY REVIEWS, 1995, 8 (04) :451-&

[8]

LIVAK KJ, 1995, PCR METH APPL, V4, P357

[9] DETECTION OF CHLAMYDIA-PNEUMONIAE AND CHLAMYDIA-PSITTACI IN SPUTUM SAMPLES BY PCR [J].

TONG, CYW ;

SILLIS, M .

JOURNAL OF CLINICAL PATHOLOGY, 1993, 46 (04) :313-317

[10] Simultaneous detection of influenza viruses A and B using real-time quantitative PCR [J].

van Elden, LJR ;

Nijhuis, M ;

Schipper, P ;

Schuurman, R ;

van Loon, AM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (01) :196-200