Nanobody-based products as research and diagnostic tools

被引：353

作者：

De Meyer, Thomas ^{[1
,2
]}

Muyldermans, Serge ^{[3
,4
]}

Depicker, Ann ^{[1
,2
]}

机构：

[1] VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[2] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium

[3] VIB, Struct Biol Res Ctr, B-1050 Brussels, Belgium

[4] Vrije Univ Brussel, Res Unit Cellular & Mol Immunol, B-1050 Brussels, Belgium

来源：

TRENDS IN BIOTECHNOLOGY | 2014年 / 32卷 / 05期

关键词：

nanobody; recombinant protein production; antibody fragment; GFP-binding protein; plant production platform; yeast production platform; SINGLE-DOMAIN ANTIBODIES; CRYSTAL-STRUCTURE; EFFICIENT EXPRESSION; TUMOR XENOGRAFTS; IN-VITRO; V-HH; FRAGMENTS; CHAIN; VHH; SELECTION;

D O I：

10.1016/j.tibtech.2014.03.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Since the serendipitous discovery 20 years ago of bona fide camelid heavy-chain antibodies, their single-domain antigen-binding fragments, known as VHHs or nanobodies, have received a progressively growing interest. As a result of the beneficial properties of these stable recombinant entities, they are currently highly valued proteins for multiple applications, including fundamental research, diagnostics, and therapeutics. Today, with the original patents expiring, even more academic and industrial groups are expected to explore innovative VHH applications. Here, we provide a thorough overview of novel implementations of VHHs as research and diagnostic tools, and of the recently evaluated production platforms for several VHHs and VHH-derived antibody formats.

引用

页码：263 / 270

页数：8

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