Functional assessment of precursors from murine bone marrow suggests a sequence of early B lineage differentiation events

被引：109

作者：

Tudor, KSRS ^{[1
]}

Payne, KJ ^{[1
]}

Yamashita, Y ^{[1
]}

Kincade, PW ^{[1
]}

机构：

[1] Oklahoma Med Res Fdn, Oklahoma City, OK 73104 USA

来源：

IMMUNITY | 2000年 / 12卷 / 03期

关键词：

D O I：

10.1016/S1074-7613(00)80186-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Most lineage marker-negative (Lin(-))TdT(+) cells from murine marrow lack CD34 but display c-kit at low density as well as IL-7R alpha and FIk-2/FIt-3 receptors. Single cells with these characteristics generated CD45RA(+)CD19(-) as well as CD19(+) lymphocytes in culture. CD45RA+CD19- marrow cells were resolved into three nonoverlapping subsets. One subset, lacking DX5 and Ly-6C antigens, yielded CD19(+) cells in culture. Further analysis demonstrated CD24 on most Lin(-)TdT(+) cells and all CD45R(+)CD19(-)DX5(-)Ly-6C(-) cells. Mac-1/CD11b was absent from these two subsets of B lineage precursors, while IL-7R alpha was retained during subsequent differentiation to a CD19(+) and stromal cell-independent stage. These findings contrast with previous descriptions of B lymphocyte precursors and suggest a sequence of early differentiation events.

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页码：335 / 345

页数：11

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