The gain of function of the p53 mutant Asp281Gly is dependent on its ability to form tetramers

被引：11

作者：

Atema, A

Chène, P

机构：

[1] Novartis, Oncol Dept, CH-4002 Basel, Switzerland

[2] Dana Farber Canc Inst, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Boston, MA 02115 USA

来源：

CANCER LETTERS | 2002年 / 185卷 / 01期

关键词：

p53; gain of function; tetramerisation; chemoresistance; chemosensitivity;

D O I：

10.1016/S0304-3835(02)00318-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The influence of tetramerisation on the properties of the p53 mutants is poorly understood. We describe here the influence of tetramerisation on the properties of the oncogenic mutant Asp281Gly. We show that despite being both nuclear the tetrameric Asp281Gly and the monomeric Asp281GlyLeu344Pro proteins have different properties: only Asp281 Gly stimulates the transcription of the multidrug resistance-1 gene promoter and induces cisplatin resistance in Saos-2 cells. Moreover, we identify a 130-kDa protein that specifically interacts with Asp281Gly but not with Asp281GlyLeu344Pro. This suggests that tetramerisation is important for the properties of the p53 mutants and that these properties might be mediated via protein-protein interactions. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：103 / 109

页数：7

共 25 条

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[2]

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