Confocal spectroscopy in microstructures

被引：29

作者：

Brinkmeier, M ^{[1
]}

Dorre, K ^{[1
]}

Riebeseel, K ^{[1
]}

Rigler, R ^{[1
]}

机构：

[1] KAROLINSKA INST,S-17177 STOCKHOLM,SWEDEN

来源：

BIOPHYSICAL CHEMISTRY | 1997年 / 66卷 / 2-3期

关键词：

fluorescence correlation spectroscopy; microstructures; single molecule detection; confocal; single molecule sequencing;

D O I：

10.1016/S0301-4622(97)00065-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Confocal instrumentation makes it possible to carry out spectroscopic measurements with a very high signal-to-background ratio. The transit of a single fluorescent molecule through the focal point of the light can be monitored with this method. The particle transport can be observed in transparent microchannels. Examples of fluorescence correlation spectroscopy and single molecule handling in microstructures are discussed. (C) 1997 Published by Elsevier Science B.V.

引用

页码：229 / 239

页数：11

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