Nanosecond electric pulses trigger actin responses in plant cells

被引：51

作者：

Berghoefer, Thomas ^{[2
]}

Eing, Christian ^{[2
]}

Flickinger, Bianca ^{[2
]}

Hohenberger, Petra ^{[1
]}

Wegner, Lars H. ^{[1
,2
]}

Frey, Wolfgang ^{[2
]}

Nick, Peter ^{[1
]}

机构：

[1] Univ Karlsruhe, Karlsruhe Inst Technol, Inst Bot 1, D-76128 Karlsruhe, Germany

[2] Forschungszentrum Karlsruhe, Karlsruhe Inst Technol, Inst Pulsed Power & Microwave Technol IHM, D-76344 Eggenstein Leopoldshafen, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 387卷 / 03期

关键词：

Actin; Endoplasmic reticulum; Microtubules; Nanosecond pulsed electrical fields; Nuclear envelope; Tobacco BY-2 cells; PLASMA-MEMBRANE; ELECTROPORATION; MICROTUBULES; MECHANISMS; APOPTOSIS; SITES; DEATH; WALL;

D O I：

10.1016/j.bbrc.2009.07.072

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have analyzed the cellular effects of nanosecond pulsed electrical fields on plant cells using fluorescently tagged marker lines in the tobacco cell line BY-2 and confocal laser scanning microscopy. We observe a disintegration of the cytoskeleton in the cell cortex, followed by contraction of actin filaments towards the nucleus, and disintegration of the nuclear envelope. These responses are accompanied by irreversible permeabilization of the plasma membrane manifest as uptake of Trypan Blue. By pretreatment with the actin-stabilizing drug phalloidin, the detachment of transvaculor actin from the cell periphery can be suppressed, and this treatment can also suppress the irreversible perforation of the plasma membrane. We discuss these findings in terms of a model, where nanosecond pulsed electric fields trigger actin responses that are key events in the plant-specific form of programmed cell death. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：590 / 595

页数：6

共 28 条

[1] Nanosecond, high-intensity pulsed electric fields induce apoptosis in human cells [J].

Beebe, SJ ;

Fox, PM ;

Rec, LJ ;

Willis, LK ;

Schoenbach, KH .

FASEB JOURNAL, 2003, 17 (09) :1493-+

[2] High affinity RGD-binding sites at the plasma membrane of Arabidopsis thaliana links the cell wall [J].

Canut, H ;

Carrasco, A ;

Galaud, JP ;

Cassan, C ;

Bouyssou, H ;

Vita, N ;

Ferrara, P ;

Pont-Lezica, R .

PLANT JOURNAL, 1998, 16 (01) :63-71

[3] Membrane electroporation theories: a review [J].

Chen, C. ;

Smye, S. W. ;

Robinson, M. P. ;

Evans, J. A. .

MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING, 2006, 44 (1-2) :5-14

[4] Nanosecond electric pulses penetrate the nucleus an enhance speckle formation [J].

Chen, Nianyong ;

Garner, Allen L. ;

Chen, George ;

Jing, Yu ;

Deng, Yuping ;

Swanson, R. James ;

Kolb, Juergen F. ;

Beebe, Stephen J. ;

Joshi, Ravindra P. ;

Schoenbach, Karl H. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 364 (02) :220-225

[5] Membrane trafficking: Intracellular highways and country roads [J].

Cheung, Alice Y. ;

de Vries, Sacco C. .

PLANT PHYSIOLOGY, 2008, 147 (04) :1451-1453

[6] Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I [J].

Coleman, ML ;

Sahai, EA ;

Yeo, M ;

Bosch, M ;

Dewar, A ;

Olson, MF .

NATURE CELL BIOLOGY, 2001, 3 (04) :339-345

[7] Plant nuclei can contain extensive grooves and invaginations [J].

Collings, DA ;

Carter, CN ;

Rink, JC ;

Scott, AC ;

Wyatt, SE ;

Allen, NS .

PLANT CELL, 2000, 12 (12) :2425-2439

[8] A role for actin in regulating apoptosis/programmed cell death: evidence spanning yeast, plants and animals [J].

Franklin-Tong, Vernonica E. ;

Gourlay, Campbell W. .

BIOCHEMICAL JOURNAL, 2008, 413 :389-404

[9]

FREY N, 2009, PLANT CELL PHYSL

[10] Plasma membrane voltage changes during nanosecond pulsed electric field exposure [J].

Frey, W ;

White, JA ;

Price, RO ;

Blackmore, PF ;

Joshi, RP ;

Nuccitelli, R ;

Beebe, SJ ;

Schoenbach, KH ;

Kolb, JF .

BIOPHYSICAL JOURNAL, 2006, 90 (10) :3608-3615

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