Origin of magnetosome membrane: Proteomic analysis of magnetosome membrane and comparison with cytoplasmic membrane

被引：112

作者：

Tanaka, Masayoshi ^{[1
]}

Okamura, Yoshiko ^{[1
]}

Arakaki, Atsushi ^{[1
]}

Tanaka, Tsuyoshi ^{[1
]}

Takeyama, Haruko ^{[1
]}

Matsunaga, Tadashi ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

来源：

PROTEOMICS | 2006年 / 6卷 / 19期

关键词：

magnetosome; Magnetospirillum magneticum AMB-1; magnetotactic bacteria; proteome analysis;

D O I：

10.1002/pmic.200500887

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Prokaryotes are known to have evolved one or more unique organelles. Although several hypotheses have been proposed concerning the biogenesis of these intracellular components, the majority of these proposals remains unclear. Magnetotactic bacteria synthesize intracellular magnetosomes that are enclosed by lipid bilayer membranes. From the identification and characterization of several surface and transmembrane magnetosome proteins, we have postulated that magnetosomes are derived from the cytoplasmic membrane (CM). To confirm this hypothesis, a comparative proteomic analysis of the magnetosome membrane (MM) and CM of the magnetotactic bacterium, Magnetospirillum magneticum AMB-1, was undertaken. Based on the whole genome sequence of M. magneticum AMB-1, 78 identified MM proteins were also found to be prevalent in the CM, several of which are related to magnetosome biosynthesis, such as Mms13, which is tightly bound on the magnetite surface. Fatty acid analysis was also conducted, and showed a striking similarity between the CM and MM profiles. These results suggest that the MM is derived from the CM.

引用

页码：5234 / 5247

页数：14

共 42 条

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