Anti-c-kit protein immunoreactive cells corresponding to the interstitial cells of Cajal in the guinea-pig small intestine

被引：79

作者：

Komuro, T

Zhou, DS

机构：

[1] Department of Basic Human Sciences, School of Human Sciences, Waseda University, Tokorozawa, Saitama 359

来源：

JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM | 1996年 / 61卷 / 02期

关键词：

c-kit; pacemaker; ultrastructure; interstitial cells of Cajal; myenteric plexus; guinea-pig;

D O I：

10.1016/S0165-1838(96)00078-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Interstitial cells of Cajal (ICC) of the guinea-pig small intestine were studied with whole-mount preparations by using the zinc iodide-osmic acid method (ZIG) and immunohistochemistry for vimentin and c-kit receptor tyrosine kinase, and by electron microscopy. The myenteric ICC visualized with ZIO staining are immunopositive to both anti-c-kit antibody (ACK-2) and anti-vimentin antibody (V9), and constitute an independent cellular network from the myenteric plexus. Those cells are characterized by many mitochondria, abundant intermediate filaments, and surface cell membranes not covered with a basal lamina. They are connected with each other by gap junctions at tips of the cytoplasmic processes. It is concluded that the myenteric ICC of the guinea-pig intestine are fibroblast-like cells and that they correspond to the c-kit expressing cells regarded as the intestinal pacemaker.

引用

页码：169 / 174

页数：6

共 29 条

[1] Cajal S. R., 1911, MALOINE PARIS, V2, P891
[2] THE PROTO-ONCOGENE C-KIT ENCODING A TRANSMEMBRANE TYROSINE KINASE RECEPTOR MAPS TO THE MOUSE W-LOCUS
CHABOT, B
STEPHENSON, DA
CHAPMAN, VM
BESMER, P
BERNSTEIN, A
[J]. NATURE, 1988, 335 (6185) : 88 - 89
[3] A COMMENTARY ON THE MORPHOLOGICAL IDENTIFICATION OF INTERSTITIAL-CELLS OF CAJAL IN THE GUT
CHRISTENSEN, J
[J]. JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM, 1992, 37 (02): : 75 - 88
[4] ORIGIN OF SLOW WAVES IN THE ISOLATED PROXIMAL COLON OF THE CAT
DU, C
CONKLIN, JL
[J]. JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM, 1989, 28 (02): : 167 - 177
[5] FAUSSONEPELLEGRINI MS, 1990, ACTA ANAT, V139, P31
[6] THE DOMINANT-WHITE SPOTTING (W) LOCUS OF THE MOUSE ENCODES THE C-KIT PROTO-ONCOGENE
GEISSLER, EN
RYAN, MA
HOUSMAN, DE
[J]. CELL, 1988, 55 (01) : 185 - 192
[7] ELECTROPHYSIOLOGY OF SMOOTH-MUSCLE OF THE SMALL-INTESTINE OF SOME MAMMALS
HARA, Y
KUBOTA, M
SZURSZEWSKI, JH
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1986, 372 : 501 - 520
[8] ELECTRICAL COUPLING AND PACEMAKER ACTIVITY IN COLONIC SMOOTH-MUSCLE
HUIZINGA, JD
SHIN, A
CHOW, E
[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (05): : C653 - C660
[9] HUIZINGA JD, 1995, NATURE, V373, P341
[10] DISTURBED INTESTINAL MOVEMENT, BILE REFLUX TO THE STOMACH, AND DEFICIENCY OF C-KIT-EXPRESSING CELLS IN WS/WS MUTANT RATS
ISOZAKI, K
HIROTA, S
NAKAMA, A
MIYAGAWA, JI
SHINOMURA, Y
XU, ZD
NOMURA, S
KITAMURA, Y
[J]. GASTROENTEROLOGY, 1995, 109 (02) : 456 - 464

← 1 2 3 →