Hyperthermia-induced antitumor activity of thermosensitive polymer modified temperature-sensitive liposomes

被引:54
作者
Han, Hee Dong
Choi, Min Soo
Hwang, Taewon
Song, Chung Kil
Seong, Hasoo
Kim, Tae Woo
Choi, Ho Suk
Shin, Byung Cheol
机构
[1] Korea Res Inst Chem Technol, Med Sci Div, Taejon 305600, South Korea
[2] Korea Univ, Grad Sch Med, Sungbuk Ku, Seoul 136791, South Korea
[3] Chungnam Nalt Univ, Dept Chem Engn, Taejon 305764, South Korea
关键词
temperature-sensitive liposomes; thermosensitive polymer; hyperthermia;
D O I
10.1002/jps.20646
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Temperature-sensitive liposomes (TS-liposomes) have been studied for chemotherapeutic purposes to enhance the release of anticancer drugs at tumor sites. In this study, we prepared poly(N-isopropylacrylamide-co-acrylamide) (PNIPAM-AAM) and polyethylene glycol (PEG)-modified TS-liposomes (PETS-liposomes). PETS-liposomes significantly increased in vitro drug release in serum compared with PEG-fixed or PNIPAM-AAM-modified liposomes. Furthermore, incorporation of both PNIPAM-AAM and PEG into PETS-liposomes enhanced the stabilities of liposomes in serum by inhibiting protein adsorption. In addition, to investigate the therapeutic efficacy of doxorubicin (DOX)-loaded PETS-liposomes, the in vivo antitumor activity of liposomes in combination with hyperthermia was evaluated in a B16F10 melanoma tumor-bearing mouse model. PETS-liposomes showed much higher levels of tumor growth inhibition than PEG-fixed or PNIPAM-AAM-modified TS-liposomes. Moreover, the antitumor activity of PETS-liposomes was enhanced significantly when they were administered in combination with hyperthermia. PETS-liposomes were found to be highly efficacious carriers for the in vivo delivery of anticancer drugs, and to have potential anticancer applications in combination with hyperthermia. (c) 2006 Wiley-Liss, Inc.
引用
收藏
页码:1909 / 1917
页数:9
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