Increased recombination intermediates and homologous integration hot spots at DNA replication origins

被引：48

作者：

Segurado, M ^{[1
]}

Gómez, M ^{[1
]}

Antequera, F ^{[1
]}

机构：

[1] Univ Salamanca, CSIC, Inst Microbiol Bioquim, Edificio Dept, Salamanca 37007, Spain

来源：

MOLECULAR CELL | 2002年 / 10卷 / 04期

关键词：

D O I：

10.1016/S1097-2765(02)00684-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the relationship between DNA replication and recombination in Schizosaccharomyces pombe using two-dimensional gel electrophoresis and functional analysis. Our results indicate that the activation of replication origins (ORIs) during the mitotic cell cycle is associated with the generation of joint DNA molecules between sister chromatids. The frequency of integration by homologous recombination was up to 50-fold higher than the genomic average within a narrow window overlapping the ars1 replication initiation site. The S. pombe rad22Delta, rhp51Delta, and rhp54Delta mutants, deficient in mitotic recombination, activate ORIs very inefficiently and accumulate abnormal replication intermediates. These results focus on the general link between replication and recombination previously found in several systems and suggest a role for recombination in the initiation of eukaryotic DNA replication.

引用

页码：907 / 916

页数：10

共 57 条

[1] Start sites of bidirectional DNA synthesis at the human lamin B2 origin [J].

Abdurashidova, G ;

Deganuto, M ;

Klima, R ;

Riva, S ;

Biamonti, G ;

Giacca, M ;

Falaschi, A .

SCIENCE, 2000, 287 (5460) :2023-2026

[2]

Aguilera A, 2000, YEAST, V16, P731, DOI 10.1002/1097-0061(20000615)16:8<731::AID-YEA586>3.0.CO

[3]

2-L

[4] CpG islands as genomic footprints of promoters that are associated with replication origins [J].

Antequera, F ;

Bird, A .

CURRENT BIOLOGY, 1999, 9 (17) :R661-R667

[5] Clustering of meiotic double-strand breaks on yeast chromosome III [J].

Baudat, F ;

Nicolas, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (10) :5213-5218

[6] Fission yeast retrotransposon Tf1 integration is targeted to 5′ ends of open reading frames [J].

Behrens, R ;

Hayles, J ;

Nurse, P .

NUCLEIC ACIDS RESEARCH, 2000, 28 (23) :4709-4716

[7] DNA replication-dependent formation of joint DNA molecules in Physarum polycephalum [J].

Bénard, M ;

Maric, C ;

Pierron, G .

MOLECULAR CELL, 2001, 7 (05) :971-980

[8] Chromosomal ARS1 has a single leading strand start site [J].

Bielinsky, AK ;

Gerbi, SA .

MOLECULAR CELL, 1999, 3 (04) :477-486

[9] RNA polymerase II and III transcription factors can stimulate DNA replication by modifying origin chromatin structures [J].

Bodmer-Glavas, M ;

Edler, K ;

Barberis, A .

NUCLEIC ACIDS RESEARCH, 2001, 29 (22) :4570-4580

[10] Direct coupling between meiotic DNA replication and recombination initiation [J].

Borde, V ;

Goldman, ASH ;

Lichten, M .

SCIENCE, 2000, 290 (5492) :806-809

← 1 2 3 4 5 6 →