Challenges and developments in tandem mass spectrometry based clinical metabolomics

被引：86

作者：

Ceglarek, Uta ^{[1
]}

Leichtle, Alexander ^{[1
]}

Bruegel, Mathias ^{[1
]}

Kortz, Linda ^{[1
]}

Brauer, Romy ^{[1
]}

Bresler, Kristin ^{[1
]}

Thiery, Joachim ^{[1
]}

Fiedler, Georg Martin ^{[1
]}

机构：

[1] Univ Hosp Leipzig, Inst Lab Med Clin Chem & Mol Diagnost, D-04103 Leipzig, Germany

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2009年 / 301卷 / 1-2期

关键词：

Clinical metabolomics; Linear ion trap; Metabolite profiling; Tandem mass spectrometry; Targeted metabolomics; MAGNETIC BEAD SEPARATION; ORGANIC-ACIDS; INBORN-ERRORS; BLOOD SPOTS; METABOLISM; CHROMATOGRAPHY; METABONOMICS; TECHNOLOGY; VALIDATION; PROTEOME;

D O I：

10.1016/j.mce.2008.10.013

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

'Clinical metabolomics' aims at evaluating and predicting health and disease risk in an individual by investigating metabolic signatures in body fluids or tissues, which are influenced by genetics, epigenetics, environmental exposures, diet, and behaviour. Powerful analytical techniques like liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) offers a rapid, effective and economical way to analyze metabolic alterations of pre-defined target metabolites in biological samples. Novel hyphenated technical approaches like the combination of tandem mass spectrometry combined with linear ion trap (QTrap mass spectrometry) combines both identification and quantification of known and unknown metabolic targets. We describe new concepts and developments of mass spectrometry based multi-target metabolome profiling in the field of clinical diagnostics and research. Particularly, the experiences from newborn screening provided important insights about the diagnostic potential of metabolite profiling arrays and directs to the clinical aim of predictive, preventive and personalized medicine by metabolomics. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：266 / 271

页数：6

共 31 条

[1] Newborn screening: Current status [J].

Arn, Pamela H. .

HEALTH AFFAIRS, 2007, 26 (02) :559-566

[2] Standardized approach to proteome profiling of human serum based on magnetic bead separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J].

Baumann, S ;

Ceglarek, U ;

Fiedler, GM ;

Lembcke, J ;

Leichtle, A ;

Thiery, J .

CLINICAL CHEMISTRY, 2005, 51 (06) :973-980

[3] Validation of the phenylalanine/tyrosine ratio determined by tandem mass spectrometry:: Sensitive newborn screening for phenylketonuria [J].

Ceglarek, U ;

Müller, P ;

Stach, B ;

Bührdel, P ;

Thiery, J ;

Kiess, W .

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2002, 40 (07) :693-697

[4] A biochemical perspective on the use of tandem mass spectrometry for newborn screening and clinical testing [J].

Chace, DH ;

Kalas, TA .

CLINICAL BIOCHEMISTRY, 2005, 38 (04) :296-309

[5] Detection and quantitation of eicosanoids via high performance liquid chromatography-electrospray ionization-mass spectrometry [J].

Deems, Raymond ;

Buczynski, Matthew W. ;

Bowers-Gentry, Rebecca ;

Harkewicz, Richard ;

Dennis, Edward A. .

LIPIDOMICS AND BIOACTIVE LIPIDS: MASS-SPECTROMETRY-BASED LIPID ANALYSIS, 2007, 432 :59-82

[6] Mass spectrometry-based metabolomics [J].

Dettmer, Katja ;

Aronov, Pavel A. ;

Hammock, Bruce D. .

MASS SPECTROMETRY REVIEWS, 2007, 26 (01) :51-78

[7] Current trends and future requirements for the mass spectrometric investigation of microbial, mammalian and plant metabolomes [J].

Dunn, Warwick B. .

PHYSICAL BIOLOGY, 2008, 5 (01)

[8] Measuring the metabolome: current analytical technologies [J].

Dunn, WB ;

Bailey, NJC ;

Johnson, HE .

ANALYST, 2005, 130 (05) :606-625

[9] Standardized peptidome profiling of human urine by magnetic bead separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J].

Fiedler, Georg Martin ;

Baumann, Sven ;

Leichtle, Alexander ;

Oltmann, Anke ;

Kase, Julia ;

Thiery, Joachim ;

Ceglarek, Uta .

CLINICAL CHEMISTRY, 2007, 53 (03) :421-428

[10]

FINGERHUT R, 2008, EUR J PEDIAT

← 1 2 3 4 →