Chondrogenesis of human bone marrow-derived mesenchymal stem cells in agarose culture

被引:116
作者
Huang, CYC
Reuben, PM
D'Ippolito, G
Schiller, PC
Cheung, HS
机构
[1] Vet Affairs Med Ctr, Geriatr Res Educ & Clin Ctr, Miami, FL 33125 USA
[2] Univ Miami, Dept Biomed Engn, Coral Gables, FL 33124 USA
[3] Vet Affairs Med Ctr, Res Serv, Miami, FL 33125 USA
来源
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY | 2004年 / 278A卷 / 01期
关键词
biomaterials; cartilage; adult stem cells; TGF-beta; 3; agarose; tissue engineering;
D O I
10.1002/ar.a.20010
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Mesenchymal stem cells derived from human bone marrow (hBM-MSCs) can differentiate into chondrogenic cells for the potential treatment of injured articular cartilage. To evaluate agarose gels as a supportive material for chondrogenesis of hBM-MSCs, this study examined chondrogenesis of hBM-MSCs in the agarose cultures. Pellet cultures were employed to confirm the chondrogenic potential of the hBM-MSCs that were used in agarose cultures. The hBM-MSCs were seeded in 2% agarose constructs at the initial cell-seeding densities of 3, 6, and 9 x 10(6) cells/ml while each of pellets was formed using 2.5 x 10(5) cells. Chondrogenesis of hBM-MSCs was induced by culturing cell-agarose constructs and pellets for 21 days in the presence of a defined medium containing transforming growth factor beta3 (TGF-beta3). The analysis of reverse transcription-polymerase chain reaction showed that hBM-MSCs of agarose and pellet cultures expressed the chondrogenic markers of collagen type II and aggrecan in the presence of TGF-beta3. The deposition of cartilage-specific macromolecules was detected in both agarose and pellet cultures by histological and immunohistochemical assessments. Chondrogenesis of hBM-MSCs in agarose gels directly correlated with the initial cell-seeding density, with the cell-agarose constructs of higher initial cell-seeding density exhibiting more cartilage-specific gene expressions. This study establishes a basic model for future studies on chondrogenesis of hBM-MSCs using the agarose cultures. (C) 2004 Wiley-Liss, Inc.
引用
收藏
页码:428 / 436
页数:9
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