A novel glycolipid and phospholipid in the purple membrane

被引:69
作者
Corcelli, A [1 ]
Colella, M
Mascolo, G
Fanizzi, FP
Kates, M
机构
[1] Univ Bari, Dipartimento Fisiol Gen & Ambientale, Bari, Italy
[2] Consortium CARSO, Ctr Canc Res, Valenzano Bari, Italy
[3] Univ Bari, Dipartimento Farmacochim, Bari, Italy
[4] CNR, IRSA, Bari, Italy
[5] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON, Canada
关键词
D O I
10.1021/bi992462z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel glycolipid of mass 1935 and a phospholipid of mass 1522 al-e the main residual lipids (along with traces of PGP-Me, S-TCD-1, and PG) specifically associated with "delipidated" bacteriorhodopsin fractions BR I and BR II, prepared by Triton X-100 treatment of purple membrane (PM)I from a genetically engineered strain (L33) of Halobacterium salinarum, and chromatography on phenyl-Sepharose CL-4B. The novel,glycolipid and phospholipid are components of the PM matrix not previously described. The TLC isolated and purified novel glycolipid and phospholipid were shown, by chemical degradation, mass spectrometry, and NMR analyses, to have the structure, respectively, of a phosphosulfoglycolipid, 3-HSO3-Galp-beta 1,6Manp-alpha 1,2Glcp-alpha 1,1-[sn-2,3-di-O-phytanylglycerol]-6-[phospho-sn-2,3-di-O-phytanylglycerol], and of a glycerol diether analogue of bisphosphatidylglycerol (cardiolipin), sn-2,3-di-O-phytanyl-1-phosphoglycerol-3-phospho-sn-2,3-di-O-phytanylglycerol.
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页码:3318 / 3326
页数:9
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