Sucrose metabolism in cyanobacteria:: sucrose synthase from Anabaena sp strain PCC 7119 is remarkably different from the plant enzymes with respect to substrate affinity and amino-terminal sequence

被引:53
作者
Porchia, AC [1 ]
Curatti, L [1 ]
Salerno, GL [1 ]
机构
[1] Consejo Nacl Invest Cient & Tecn, PROBIOP, Fdn Invest Biol Aplicadas, Ctr Invest Biol, RA-7600 Mar Del Plata, Argentina
关键词
Anabaena sp (sucrose metabolism); cyanobacterium; sucrose; sucrose synthase;
D O I
10.1007/s004250050651
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The pathway of sucrose metabolism in cyanobacteria is just starting to be elucidated. The present study describes the first isolation and biochemical characterization of a prokaryotic sucrose synthase (SS, EC 2.4.1.13). Two SS forms(SS-I and SS-II) were detected in Anabaena sp. strain PCC 7119. The isoform SS-II was purified 457-fold and its amino-terminal portion sequenced. Substrate specificity, kinetic constants, native protein and subunit molecular masses, and the effect of different ions and metabolites were studied for SS-II. Anabaena SS was shown to be a tetramer with a 92-kDa polypeptide that was recognized by maize SS polyclonal antibodies. Some striking differences from plant enzymes were demonstrated with respect to substrate affinities, regulation by metal ions and ATP, and the amino-acid sequence of the N-terminal region.
引用
收藏
页码:34 / 40
页数:7
相关论文
共 36 条
  • [1] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [2] A MEMBRANE-ASSOCIATED FORM OF SUCROSE SYNTHASE AND ITS POTENTIAL ROLE IN SYNTHESIS OF CELLULOSE AND CALLOSE IN PLANTS
    AMOR, Y
    HAIGLER, CH
    JOHNSON, S
    WAINSCOTT, M
    DELMER, DP
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) : 9353 - 9357
  • [3] [Anonymous], ENCY PLANT PHYSL
  • [4] Black CC, 1995, CUR TOP PL, V14, P49
  • [5] GENETIC-CONTROL OF SUCROSE SYNTHETASE IN MAIZE ENDOSPERM
    CHOUREY, PS
    [J]. MOLECULAR AND GENERAL GENETICS, 1981, 184 (03): : 372 - 376
  • [6] COMPARATIVE INVESTIGATIONS ON THE DISTRIBUTION OF SUCROSE SYNTHASE ACTIVITY AND INVERTASE ACTIVITY WITHIN GROWING, MATURE AND OLD LEAVES OF SOME C-3 AND C-4 PLANT-SPECIES
    CLAUSSEN, W
    LOVEYS, BR
    HAWKER, JS
    [J]. PHYSIOLOGIA PLANTARUM, 1985, 65 (03) : 275 - 280
  • [7] Copeland L, 1990, METHODS PLANT BIOCH, V3, P73
  • [8] Sucrose-phosphate synthase from Synechocystis sp. Strain PCC 6803:: Identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli
    Curatti, L
    Folco, E
    Desplats, P
    Abratti, G
    Limones, V
    Herrera-Estrella, L
    Salerno, G
    [J]. JOURNAL OF BACTERIOLOGY, 1998, 180 (24) : 6776 - 6779
  • [9] Purification, characterization and physiological role of sucrose synthase in the pea seed coat (Pisum sativum L)
    Dejardin, A
    Rochat, C
    Maugenest, S
    Boutin, JP
    [J]. PLANTA, 1997, 201 (02) : 128 - 137
  • [10] SUCROSE METABOLISM IN GREEN-ALGAE .1. PRESENCE OF SUCROSE SYNTHETASE AND SUCROSE PHOSPHATE SYNTHETASE
    DURAN, WR
    PONTIS, HG
    [J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 1977, 16 (2-3) : 149 - 152