Construction of BAC library for the amphibian Xenopus tropicalis

被引：4

作者：

Ishii, Y

Asakawa, S

Taguchi, Y

Ishibashi, S

Yagi, T

Shimizu, N

机构：

[1] Keio Univ, Sch Med, Dept Mol Biol, Shinjuku Ku, Tokyo 1608582, Japan

[2] Osaka Univ, Grad Sch Frontier Biosci, KOKORO Biol Grp, Labs Integrated Biol, Suita, Osaka 5650871, Japan

[3] NAIST, Grad Sch Biol Sci, Nara 6300101, Japan

[4] Natl Inst Physiol Sci, Lab Neurobiol & Behav Genet, Okazaki, Aichi 4448585, Japan

来源：

GENES & GENETIC SYSTEMS | 2004年 / 79卷 / 01期

关键词：

amphibian; BAC; genomic library; Pcdh gamma; Xenopus tropicalis;

D O I：

10.1266/ggs.79.49

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Xenopus tropicalis has become an alternative model to the amphibian Xenopus laevis because it is better suited for genetic and genonnic studies. We have constructed a genomic BAC library consisting of over 100,000 clones from sperm of Xenopus tropicalis. Analysis by pulsed field gel electrophoresis of representative BAC clones indicated the average size of insert DNA to be 100 kb, and we estimated the library covers 6 times the Xenopus tropicalis genome of 1.7 x 10(9) base pairs. To evaluate the BAC library, we attempted to isolate BAC clones which contain a protocadherin gamma(Pcdhgamma) gene and found that the isolated BAC clones are assembled as two separate contigs. This result suggests the presence of at least two clusters for the Pcdhgamma gene in the genome of X. tropicalis.

引用

页码：49 / 51

页数：3

共 14 条

[1] Frog genetics:: Xenopus tropicalis jumps into the future [J].