Kinetic parameters of two rice alpha-amylase isozymes for oligosaccharide degradation

被引：8

作者：

Terashima, M ^{[1
]}

Hayashi, N ^{[1
]}

Thomas, BR ^{[1
]}

Rodriguez, RL ^{[1
]}

Katoh, S ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,SECT MOLEC & CELLULAR BIOL,DAVIS,CA 95616

来源：

PLANT SCIENCE | 1996年 / 116卷 / 01期

关键词：

rice; alpha-amylase; kinetic constant; oligosaccharide; carbon metabolite signal; action pattern;

D O I：

10.1016/0168-9452(96)04373-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rice alpha-amylase isozymes Amy1A and Amy3D have distinct kinetic parameters and product profiles, For Amy3D, Michaelis constants (K-m) decrease, and molecular activities (k(cat)) increase with the chain length of the substrates from maltose to maltoheptaose. Although Amy1A has a lower K-m than Amy3D, the k(cat) of Amy1A was much lower than that of Amy3D. Amy1A produces only negligible amounts of glucose, while Amy3D produces glucose as a major product. Glucose regulates the expression of a number of genes in developing seedlings, so Amy3D may have a unique regulatory role in control of seedling development.

引用

页码：9 / 14

页数：6

共 16 条

[1] BARLEY MALT-ALPHA-AMYLASE - PURIFICATION, ACTION PATTERN, AND SUBSITE MAPPING OF ISOZYME-1 AND 2 MEMBERS OF THE ISOZYME-2 SUBFAMILY USING PARA-NITROPHENYLATED MALTOOLIGOSACCHARIDE SUBSTRATES [J].

AJANDOUZ, EH ;

ABE, J ;

SVENSSON, B ;

MARCHISMOUREN, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1159 (02) :193-202

[2] MOLECULAR AND CELLULAR BIOLOGY ASSOCIATED WITH ENDOSPERM MOBILIZATION IN GERMINATING CEREAL-GRAINS [J].

FINCHER, GB .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1989, 40 :305-346

[3] CLASSIFICATION AND EVOLUTION OF ALPHA-AMYLASE GENES IN PLANTS [J].

HUANG, N ;

STEBBINS, GL ;

RODRIGUEZ, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) :7526-7530

[4] METABOLIC-REGULATION OF ALPHA-AMYLASE GENE-EXPRESSION IN TRANSGENIC CELL-CULTURES OF RICE (ORYZA-SATIVA L) [J].

HUANG, N ;

CHANDLER, J ;

THOMAS, BR ;

KOIZUMI, N ;

RODRIGUEZ, RL .

PLANT MOLECULAR BIOLOGY, 1993, 23 (04) :737-747

[5] RECENT ADVANCES IN KNOWLEDGE OF STARCH STRUCTURE [J].

IMBERTY, A ;

BULEON, A ;

TRAN, V ;

PEREZ, S .

STARCH-STARKE, 1991, 43 (10) :375-384

[6]

KATOH S, 1995, APPL MICROBIOL BIOT, V43, P871, DOI 10.1007/s002530050498

[7]

KATOH S, 1994, APPL MICROBIOL BIOT, V42, P36, DOI 10.1007/s002530050213

[8]

KNUTSON CA, 1993, CEREAL CHEM, V70, P534

[9] THE ACTION OF GERMINATED BARLEY ALPHA-AMYLASES ON LINEAR MALTODEXTRINS [J].

MACGREGOR, AW ;

MORGAN, JE ;

MACGREGOR, EA .

CARBOHYDRATE RESEARCH, 1992, 227 :301-313

[10] RELATIONSHIPS BETWEEN STRUCTURE AND ACTIVITY IN THE ALPHA-AMYLASE FAMILY OF STARCH-METABOLIZING ENZYMES [J].

MACGREGOR, EA .

STARCH-STARKE, 1993, 45 (07) :232-237

← 1 2 →