Illuminating the role of caspases during Drosophila oogenesis

被引:62
作者
Mazzalupo, S.
Cooley, L.
机构
[1] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06520 USA
[2] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06520 USA
[3] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
关键词
oogenesis; nurse cell; caspase; apoptosis; germline; CFP; YFP; Drosophila melanogaster; phagocytosis; follicle cell;
D O I
10.1038/sj.cdd.4401892
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cell death is a prominent feature of animal germline development. In Drosophila, the death of 15 nurse cells is linked to the development of each oocyte. In addition, females respond to poor environmental conditions by inducing egg chamber death prior to yolk uptake by the oocyte. To study these two forms of cell death, we analyzed caspase activity in the germline by expressing a transgene encoding a caspase cleavage site flanked by cyan fluorescent protein and yellow fluorescent protein. When expressed in ovaries undergoing starvation-induced apoptosis, this construct was an accurate reporter of caspase activity. However, dying nurse cells at the end of normal oogenesis showed no evidence of cytoplasmic caspase activity. Furthermore, although expression of the caspase inhibitors p35 or Drosophila inhibitor of apoptosis protein 1 blocked starvation-induced death, it did not affect normal nurse cell death or overall oogenesis in well-fed females. Our data suggest that caspases play no role in developmentally programmed nurse cell death.
引用
收藏
页码:1950 / 1959
页数:10
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