Ultrasensitive biosensors

被引：26

作者：

Bier, FF

EhrentreichForster, E

Scheller, FW

Makower, A

Eremenko, A

Wollenberger, U

Bauer, CG

Pfeiffer, D

Michael, N

机构：

[1] RES CTR MOL DIAGNOST & THERAPY,DEPT BIOSENSORS,MOSCOW 113149,RUSSIA

[2] BST BIO SENSOR TECHNOL GMBH BERLIN,D-13156 BERLIN,GERMANY

[3] RES INST MOL PHARMACOL,D-10315 BERLIN,GERMANY

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1996年 / 33卷 / 1-3期

关键词：

biosensor; enzymatic amplifiers; cycling electrodes; catecholamines; immunoassay;

D O I：

10.1016/0925-4005(96)01946-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Bi-enzymatic amplifiers using cyclic reactions consisting of NADH independent dehydrogenases and phenol oxidases were found to be highly sensitive to several catecholamines and p-aminophenol. Amplification factors range from several 100 to up to 10 000 depending on substrate and enzyme loading. The detection limit for catecholamines was subnanomolar. An electrode based on tyrosinase/glucose dehydrogenase responds to peptides containing tyrosine. Applications to monitoring of cell activities as well as to immunodetection are described. Immunoassays were constructed by combining the amplification cycle with a suitable enzyme label, of which p-aminophenoi is the product of the enzymatic reaction. Alkaline phosphatase and beta-galactosidase were tested as labels for immunoglobulin G from goat (gtIgG), as well as adaption to hapten determination in a displacement format.

引用

页码：5 / 12

页数：8

共 25 条

[1]

Bier FF, 1996, FRESEN J ANAL CHEM, V354, P861

[2]

BIER FF, 1996, IN PRESS ANAL CHIM A

[3]

GHINDILIS AL, 1995, PHARMAZIE, V50, P599

[4] DETERMINATION OF P-AMINOPHENOL AND CATECHOLAMINES AT PICOMOLAR CONCENTRATIONS BASED ON RECYCLING ENZYME AMPLIFICATION [J].