Preservation of human islet cell functional mass by anti-oxidative action of a novel SOD mimic compound

被引:138
作者
Bottino, R
Balamurugan, AN
Bertera, S
Pietropaolo, M
Trucco, M
Piganelli, JD
机构
[1] Univ Pittsburgh, Childrens Hosp Pittsburgh, Sch Med,Dept Pediat, Rangos Res Ctr,Diabet Inst,Div Immunogenet, Pittsburgh, PA 15213 USA
[2] Univ Pittsburgh, Sch Med, Thomas E Starlz Transplantat Inst, Dept Surg, Pittsburgh, PA 15213 USA
关键词
D O I
10.2337/diabetes.51.8.2561
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The most commonly used technical approach to isolate human pancreatic islets intended for allotransplants generates a product that is hampered by mechanical and chemical insults, which diamatically reduce the mass of viable and functional transplantable cells. We tested a novel class of antioxidant chemical compounds (SOD mimics: AEOL10113 and AEOL16150) to protect human islets from oxidative stress in order to improve the preservation of the isolated tissue. Addition of SOD mimic in culture, after isolation, allowed for the survival of a significantly higher islet cell mass.. Functional behavior and phenotypic cell characteristics of the SOD-treated islet preparations were preserved, as was the capacity to normalize diabetic mice, even I when a marginal mass of islets was transplanted. The addition of SOD mimic during isolation, before culture, further reduced early cell loss. These results indicate that prompt interventions aimed at blocking oxidative stress can improve human islet survival, preserving a functional islet mass two- to threefold larger than the one usually obtained without adding any antioxidant compound. The ability to preserve functional islets without a dramatic loss represents a major advantage considering the scarce availability of islet tissue for clinical transplantation.
引用
收藏
页码:2561 / 2567
页数:7
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