Selection of proteins and peptides from libraries displayed on filamentous bacteriophage

被引：24

作者：

McGregor, D ^{[1
]}

机构：

[1] SCOTGEN LTD,ABERDEEN,SCOTLAND

来源：

MOLECULAR BIOTECHNOLOGY | 1996年 / 6卷 / 02期

关键词：

V-H; antibody heavy chain variable sequence region; V-L; antibody light chain variable sequence region; Fab; intact light chain (V-L and constant region domain [C-L]); associated with a truncated heavy chain composed of the V-H; domain and the first constant region domain (C(H)1); scFv; V-H adn V-L domains linked as a; single expression unit by a flexible linker; CDR; (complementarity determining region); region of hypervariable sequence within; the antibody variable domain;

D O I：

10.1007/BF02740770

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This article attempts to review recent developments in the rapidly developing field of phage display libraries. The current state of peptide, antibody, and cDNA libraries, as well as current and future applications of phage display libraries are discussed. The main focus of the article is on the methods for selecting binding ligands against targets in a variety of different formats. These include solid phase and in-solution selection methods, and the strategies used to select for higher affinity, and binding ligands against impure and cellular target proteins.

引用

页码：155 / 162

页数：8

共 55 条

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