Ribosomal protein gene transcription in Saccharomyces cerevisiae shows a biphasic response to nutritional changes

被引:29
作者
Griffioen, G [1 ]
Laan, RJ [1 ]
Mager, WH [1 ]
Planta, RJ [1 ]
机构
[1] FREE UNIV AMSTERDAM,IMBW,BIOCTR,DEPT BIOCHEM & MOL BIOL,NL-1081 HV AMSTERDAM,NETHERLANDS
来源
MICROBIOLOGY-UK | 1996年 / 142卷
关键词
Saccharomyces cerevisiae; nutritional signalling; ribosomal protein gene;
D O I
10.1099/13500872-142-8-2279
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Nutrients are major determinants of ribosomal protein (rp-) gene transcription in Saccharomyces cerevisiae. In order to investigate the molecular mechanisms underlying this nutritional control, yeast mutants that display defects in the glucose upshift response of rp-gene transcription were isolated. Interestingly, although growth of these mutants on glucose-containing medium was severely affected an initial increase in rp-gene transcription by nutritional upshift was still observed. However, at later time points, rp-mRNA levels decreased strongly. Various other types of severe growth limitation also did not prevent the initial upshift in transcription. The results suggest that the glucose upshift response of rp-gene transcription comprises two phases: an initial, transient response independent of the actual growth potential, and a sustained response which is dependent on growth and requires both glucose and adequate nitrogen sources. Previously, it was found that protein kinase A (Pka) mediates the initial upshift response, without the need for regulation of Pka activity by cAMP. The present data substantiate that, besides the RAS/adenylate cyclase pathway, an alternative pathway through Pka regulates rp-gene transcription. In addition, evidence is presented that the sustained response does not require Pka activity. Based on these results, taken together, a model is proposed in which rp-gene transcription is dynamically regulated by multiple signal transduction pathways.
引用
收藏
页码:2279 / 2287
页数:9
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