A methyl group at C7 of 11-cis-retinal allows chromophore formation but affects rhodopsin activation

被引：4

作者：

Bosch, Laia

Cordomi, Arnau

Dominguez, Marta

Toledo, Darwin

Morillo, Margarita

Perez, Juan J.

Alvarez, Rosana

de Lera, Angel R.

Garriga, Pere ^{[1
]}

机构：

[1] Univ Politecn Catalunya, Dept Engn Quim, Terrassa 08222, Catalonia, Spain

[2] Univ Politecn Catalunya, Dept Engn Quim, E-08028 Barcelona, Catalonia, Spain

[3] Univ Vigo, Fac Quim, Dept Quim Organ, Vigo 36310, Spain

来源：

VISION RESEARCH | 2006年 / 46卷 / 27期

关键词：

retinal analogues; chromophore regeneration; photobleaching; rhodopsin photoactivation; transducin activation;

D O I：

10.1016/j.visres.2006.07.031

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

The newly synthesized 11-cis-7-methylretinal can form an artificial visual pigment with kinetic and spectroscopic properties similar to the native pigment in the dark-state. However, its photobleaching behavior is altered, showing a Meta I-like photoproduct. This behavior reflects a steric constraint imposed by the 7-methyl group that affects the conformational change in the binding pocket as a result of retinal photoisomerization. Transducin activation is reduced, when compared to the native pigment with 11-cis-retinal. Molecular dynamics simulations suggest coupling of the C7 methyl group and the beta-ionone ring with Met207 in transmembrane helix 5 in agreement with recent experimental results. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：4472 / 4481

页数：10

共 70 条

[1]

PHOTOLYSIS INTERMEDIATES OF THE ARTIFICIAL VISUAL PIGMENT CIS-5,6-DIHYDRO-ISORHODOPSIN [J].