The preparation of fluorescence-quenched probes for use in the characterization of human factor Xa substrate binding domains

被引：7

作者：

Bromfield, KM

Cianci, J

Duggan, PJ ^{[1
]}

机构：

[1] Monash Univ, Sch Chem, Clayton, Vic 3800, Australia

[2] Monash Univ, Ctr Green Chem, Clayton, Vic 3800, Australia

[3] CSIRO Mol Sci, Clayton, Vic 3169, Australia

来源：

MOLECULES | 2004年 / 9卷 / 06期

关键词：

solid phase peptide synthesis; parallel synthesis; fluorescence-quenched substrates; protease; factor Xa;

D O I：

10.3390/90600427

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation and characterization by LCMS of a library of 55 fluorescence-quenched peptides is described. The peptides bear a terminal anthranilamide fluorophore and a penultimate 2,4-dinitrophenyl-L-lysine quencher, and will be used to probe the substrate binding domain of the human blood coagulation enzyme, factor Xa.

引用

收藏

页码：427 / 439

页数：13

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