A comparison of ecotoxicological tests

被引:13
作者
Botsford, JL [1 ]
机构
[1] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA
来源
ATLA-ALTERNATIVES TO LABORATORY ANIMALS | 2002年 / 30卷 / 05期
关键词
bacterial indicator; ecotoxicity testing; indicator organism; Sinorhizobium meliloti;
D O I
10.1177/026119290203000507
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
A simple, inexpensive and rapid method of determining toxicity by using a bacterium as the indicator organism was developed and compared with 23 other tests. The average correlation coefficient when comparing these 23 tests with the present test was 0.800, ranging from 0.580 to 0.950. Eleven of the tests were compared in detail by using 35 of the chemicals on the Multicentre Evaluation of In Vitro Cytotoxicity list of test chemicals. Comparing results from the present test with test results for these 35 chemicals with Microtox(TM), Biotox(TM), Daphnia magna, rat hepatocytes and ascites tumour cell resulted in correlation coefficients ranging from 0.871 to 0.933. Comparisons of the test data with rodent LD50 values, human lethal dose estimates from autopsies and human lethal doses obtained from the literature provided correlation coefficients ranging from 0.580 to 0.770, indicating that the test compares less favourably with these methods. This test provides data comparable to data from other ecotoxicological tests.
引用
收藏
页码:539 / 550
页数:12
相关论文
共 35 条
[1]  
BITTON G, 1986, TOXICITY TESTING USI
[2]   A simple assay for toxic chemicals using a bacterial indicator [J].
Botsford, JL .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1998, 14 (03) :369-376
[3]  
Botsford JL, 1999, ENVIRON TOXICOL, V14, P285, DOI 10.1002/(SICI)1522-7278(199905)14:2<285::AID-TOX10>3.3.CO
[4]  
2-7
[5]   Assay for toxic chemicals using bacteria [J].
Botsford, JL ;
Rivera, J ;
Navarez, J ;
Riley, R ;
Wright, T ;
Baker, R .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1997, 59 (06) :1000-1009
[6]   Role of EDTA in a simple method for determining toxicity using a bacterial indicator organism [J].
Botsford, JL .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2000, 16 (04) :353-359
[7]  
BULLICH AA, 1990, J BIOLUMINESCENCE CH, V5, P761
[8]   COMPARATIVE ACUTE TOXICITY OF THE FIRST 50 MULTICENTER EVALUATION OF IN-VITRO CYTOTOXICITY CHEMICALS TO AQUATIC NON-VERTEBRATES [J].
CALLEJA, MC ;
PERSOONE, G ;
GELADI, P .
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1994, 26 (01) :69-78
[9]  
CALLEJA MC, 1993, ATLA-ALTERN LAB ANIM, V21, P330
[10]  
Clemedson C, 1996, ATLA-ALTERN LAB ANIM, V24, P273