Mercury(II) sorption to two Florida Everglades peats: Evidence for strong and weak binding and competition by dissolved organic matter released from the peat

The binding of mercury(II) to two peats from Florida Everglades sites with different rates of mercury methylation was measured at pH 6.0 and 0.01 M ionic strength. The mercury(II) sorption isotherms, measured over a total mercury(II) range of 10(-7.4) to 10(-3.7) M, showed the competition for mercury(II) between the peat and dissolved organic matter released from the peat and the existence of strong and weak binding sites for mercury(II). Binding was portrayed by a model accounting for strong and weak sites on both the peat and the released DOM. The conditional binding constants (for which the ligand concentration was set as the concentration of reduced sulfur in the organic matter as measured by X-ray absorption near-edge structure spectroscopy) determined for the strong sites on the two peats were similar (K-peat,K-s = 10(21.8+/-01) and 10(22.0+/-0.1) M-1), but less than those determined for the DOM strong sites (K-dom,K-s = 10(22.8+/-0.1) and 10(23.2+/-0.1) M-1), resulting in mercury(II) binding by the DOM at low mercury(II) concentrations. The magnitude of the strong site binding constant is indicative of mercury(II) interaction with organic thiol functional groups. The conditional binding constants determined for the weak peat sites (K-peat,K-w = 10(11.5+/-0.1) and 10(11.8+/-0.1) M-1) and weak DOM sites (K-dom,K-w = 10(8.7+/-3.0) and 10(7.3+/-4.5) M-1) were indicative of mercury(II) interaction with carboxyl and phenol functional groups.