Synthesis of 5-methyl-2'-O-deoxycytidine analogs to determine monoclonal antibody specificity in the recognition of the 6-(p-bromobenzoylamino)caproyl radical.

被引：6

作者：

RodriguezTanty, C ^{[1
]}

HigginsonClarke, D ^{[1
]}

Hernandez, M ^{[1
]}

Perez, R ^{[1
]}

VelezCastro, H ^{[1
]}

Riveron, AM ^{[1
]}

Macias, A ^{[1
]}

机构：

[1] NATL CTR SCI RES, DEPT MOL BIOL, HAVANA 6880, CUBA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1997年 / 16卷 / 04期

关键词：

D O I：

10.1080/07328319708001362

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eight new p-bromobenzoyl derivatives of 5-methyl-2'-O-deoxycytidine analogs, substituted at the Lt-position, were synthesized. The best conditions for obtaining 5-methyl-4-N-aminoalkyl-2'-O-deoxycytidine from 3',5'-di-O-acetyl-4-(1,2,4-triazol-1-yl)-2'-O-deoxythymidine were studied. The nucleoside analogs were used to identify the fragment of the 6-(p-bromobenzoylamino)caproyl radical that binds to the monoclonal antibody obtained against it and to define an affinity scale of monoclonal antibody against them.

引用

页码：455 / 467

页数：13

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