EGCG mediated downregulation of NF-AT and macrophage infiltration in experimental hepatic steatosis

被引:10
作者
Krishnan, Thulasi Raman [1 ]
Velusamy, Prema [1 ]
Srinivasan, Ashokkumar [1 ]
Ganesan, Thellamudhu [1 ]
Mangaiah, Suresh [1 ]
Narasimhan, Kishorekumar [1 ]
Chakrapani, Lakshmi Narasimhan [1 ]
Thanka, J. [2 ]
Walter, Charles Emmanuel Jebaraj [3 ]
Durairajan, Sankari [3 ]
Saravanabavan, Sanddhya Nathakattur [4 ]
Periandavan, Kalaiselvi [1 ]
机构
[1] Univ Madras, DR ALM PG IBMS, Dept Med Biochem, Chennai 600113, Tamil Nadu, India
[2] Sri Ramachandra Univ, Dept Pathol, Chennai, Tamil Nadu, India
[3] Sri Ramachandra Univ, Dept Biotechnol, Fac Res & Bio Med Sci, Chennai, Tamil Nadu, India
[4] Univ Madras, Dept Pathol, DR ALM PG IBMS, Chennai, Tamil Nadu, India
关键词
Hypercholesterolemia; High cholesterol diet; Hepatic damage; Macrophage infiltration; Green tea; Epigallocatechin-3-gallate; NECROSIS-FACTOR-ALPHA; GREEN TEA; KAPPA-B; ADHESION MOLECULES; P-SELECTIN; TRANSCRIPTIONAL REGULATION; NEUTROPHIL INFILTRATION; INFLAMMATORY CYTOKINES; LEUKOCYTE RECRUITMENT; ICAM-1; EXPRESSION;
D O I
10.1016/j.exger.2014.05.008
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
030301 [社会学]; 100201 [内科学];
摘要
Scope: Increased fat consumption in industrialized countries has resulted in hepatic steatosis that upregulates atherogenic aspirant genes, leading to atherosclerosis and mortality. Although extensive studies have been carried out to elucidate the atheroprotective efficacy of epigallocatechin-3-gallate (EGCG), the effect of EGCG on hepatic steatosis has not been studied comprehensively. Hence, the current study was designed to find out the effect of EGCG on hepatic events that prelude atherosclerosis with special reference to macrophage infiltration. Methods and results: Male albino rats of Wistar strain were used in this study. Basic biochemical assays along with the protein expression of CAMs, NF-kappa B, TNF-alpha and NF-AT were assayed in the current study. EGCG supplementation significantly reverted the alterations in both biochemical and histological parameters and is shown to reduce the TNF-alpha mediated NF-AT expression and thereby its downstream targets like ICAM-1 and E-selectin expression to a greater extent than NF-kappa B mediated downstream targets like VCAM-1 and P-selectin in hypercholesterolemic rat liver. Conclusion: Our results suggest that EGCG influences the early events of atherosclerosis that occur; thereby modulating the NF-AT pathway and thereby mitigating the hypercholesterolemic stress. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:96 / 103
页数:8
相关论文
共 70 条
[1]
Mechanisms of Immune-Mediated Liver Injury [J].
Adams, David H. ;
Ju, Cynthia ;
Ramaiah, Shashi K. ;
Uetrecht, Jack ;
Jaeschke, Hartmut .
TOXICOLOGICAL SCIENCES, 2010, 115 (02) :307-321
[2]
Epigallocatechin gallate accelerates healing of indomethacin-induced stomach ulcers in mice [J].
Adhikary, Biplab ;
Yadav, Sudhir K. ;
Bandyopadhyay, Sandip K. ;
Chattopadhyay, Subrata .
PHARMACOLOGICAL REPORTS, 2011, 63 (02) :527-536
[3]
Role of cell adhesion molecules in leukocyte recruitment in the liver and gut [J].
Ala, A ;
Dhillon, AP ;
Hodgson, HJ .
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 2003, 84 (01) :1-16
[4]
Non-alcoholic fatty liver disease [J].
Angulo, P ;
Lindor, KD .
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2002, 17 :S186-S190
[5]
NFAT dysregulation by increased dosage of DSCR1 and DYRK1A on chromosome 21 [J].
Arron, Joseph R. ;
Winslow, Monte M. ;
Polleri, Alberto ;
Chang, Ching-Pin ;
Wu, Hai ;
Gao, Xin ;
Neilson, Joel R. ;
Chen, Lei ;
Heit, Jeremy J. ;
Kim, Seung K. ;
Yamasaki, Nobuyuki ;
Miyakawa, Tsuyoshi ;
Francke, Uta ;
Graef, Isabella A. ;
Crabtree, Gerald R. .
NATURE, 2006, 441 (7093) :595-600
[6]
Chronic Plus Binge Ethanol Feeding Synergistically Induces Neutrophil Infiltration and Liver Injury in Mice: A Critical Role for E-selectin [J].
Bertola, Adeline ;
Park, Ogyi ;
Gao, Bin .
HEPATOLOGY, 2013, 58 (05) :1814-1823
[7]
CCN1 expression in hepatocytes contributes to macrophage infiltration in nonalcoholic fatty liver disease in mice [J].
Bian, Zhaolian ;
Peng, Yanshen ;
You, Zhengrui ;
Wang, Qixia ;
Miao, Qi ;
Liu, Yuan ;
Han, Xiaofeng ;
Qiu, Dekai ;
Li, Zhiping ;
Ma, Xiong .
JOURNAL OF LIPID RESEARCH, 2013, 54 (01) :44-54
[8]
Beneficial effects of green tea -: A review [J].
Cabrera, C ;
Artacho, R ;
Giménez, R .
JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION, 2006, 25 (02) :79-99
[9]
A field of myocardial-endocardial NFAT signaling underlies heart valve morphogenesis [J].
Chang, CP ;
Neilson, JR ;
Bayle, JH ;
Gestwicki, JE ;
Kuo, A ;
Stankunas, K ;
Graef, IA ;
Crabtree, GR .
CELL, 2004, 118 (05) :649-663
[10]
A green tea-derived polyphenol, epigallocatechin-3-gallate, inhibits IκB kinase activation and IL-8 gene expression in respiratory epithelium [J].
Chen, PC ;
Wheeler, DS ;
Malhotra, V ;
Odoms, K ;
Denenberg, AG ;
Wong, HR .
INFLAMMATION, 2002, 26 (05) :233-241