Dps protects cells against multiple stresses during stationary phase

被引:285
作者
Nair, S [1 ]
Finkel, SE [1 ]
机构
[1] Univ So Calif, Mol & Computat Biol Program, Dept Sci Biol, Los Angeles, CA 90089 USA
关键词
D O I
10.1128/JB.186.13.4192-4198.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Dps, the nonspecific DNA-binding protein from starved cells, is the most abundant protein in stationary-phase Escherichia coli. Dps homologs are found throughout the bacteria and in at least one archaeal species. Dps has been shown to protect cells from oxidative stress during exponential-phase growth. During stationary phase, Dps organizes the chromosome into a highly ordered, stable nucleoprotein complex called the biocrystal. We show here that Dps is required for long-term stationary-phase viability under competitive conditions and that dps mutants have altered lag phases compared to wild-type cells. We also show that during stationary phase Dps protects the cell not only from oxidative stress but also from UV and gamma irradiation, iron and copper toxicity, thermal stress, and acid and base shock. The protective roles of Dps are most likely achieved through a combination of functions associated with the protein-DNA binding and chromosome compaction, metal chelation, ferroxidase activity, and regulation of gene expression.
引用
收藏
页码:4192 / 4198
页数:7
相关论文
共 30 条
[1]  
Adamcík J, 2002, ELECTROPHORESIS, V23, P3300, DOI 10.1002/1522-2683(200210)23:19<3300::AID-ELPS3300>3.0.CO
[2]  
2-Q
[3]   A NOVEL DNA-BINDING PROTEIN WITH REGULATORY AND PROTECTIVE ROLES IN STARVED ESCHERICHIA-COLI [J].
ALMIRON, M ;
LINK, AJ ;
FURLONG, D ;
KOLTER, R .
GENES & DEVELOPMENT, 1992, 6 (12B) :2646-2654
[4]   THE DPS PROMOTER IS ACTIVATED BY OXYR DURING GROWTH AND BY IHF AND A SIGMA(S) IN STATIONARY-PHASE [J].
ALTUVIA, S ;
ALMIRON, M ;
HUISMAN, G ;
KOLTER, R ;
STORZ, G .
MOLECULAR MICROBIOLOGY, 1994, 13 (02) :265-272
[5]   Gene activation by histone and factor acetyltransferases [J].
Berger, SL .
CURRENT OPINION IN CELL BIOLOGY, 1999, 11 (03) :336-341
[6]  
BORENSTEIN LA, 1988, J IMMUNOL, V140, P2415
[7]   INCREASED SENSITIVITY TO GAMMA-IRRADIATION IN BACTERIA LACKING PROTEIN HU [J].
BOUBRIK, F ;
ROUVIEREYANIV, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (09) :3958-3962
[8]   A novel non-heme iron-binding ferritin related to the DNA-binding proteins of the Dps family in Listeria innocua [J].
Bozzi, M ;
Mignogna, G ;
Stefanini, S ;
Barra, D ;
Longhi, C ;
Valenti, P ;
Chiancone, E .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (06) :3259-3265
[9]   Fine tangled pili expressed by Haemophilus ducreyi are a novel class of pili [J].
Brentjens, RJ ;
Ketterer, M ;
Apicella, MA ;
Spinola, SM .
JOURNAL OF BACTERIOLOGY, 1996, 178 (03) :808-816
[10]   O2 evolution in the Fenton reaction [J].
Buda, F ;
Ensing, B ;
Gribnau, MCM ;
Baerends, EJ .
CHEMISTRY-A EUROPEAN JOURNAL, 2003, 9 (14) :3436-3444