Separation of salidroside from Rhodiola crenulata by high-speed counter-current chromatography

被引：52

作者：

Han, X

Zhang, TY

Wei, Y

Cao, XL

Ito, Y

机构：

[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

[2] Beijing Inst New Technol Applicat, Beijing Res Ctr Separat & Purificat Technol Nat P, Beijing 100035, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 971卷 / 1-2期

关键词：

counter-current chromatography; Rhodiola crenulata; salidroside;

D O I：

10.1016/S0021-9673(02)01041-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate-n-butanol-water (1:4:5, v/v) in the first run and chloroform-methanol-isopropanol-water (5:6:1:4) in the second run. The method yielded 21.9 mg of salidroside from 1.216 g of the crude sample at 98% purity determined by HPLC analyses. Identification was performed by H-1 NMR, C-13 NMR, and MS.

引用

页码：237 / 241

页数：5