Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey and subsequent plant regeneration

被引：85

作者：

Chen, JT ^{[1
]}

Chang, C ^{[1
]}

Chang, WC ^{[1
]}

机构：

[1] Acad Sinica, Inst Bot, Taipei 115, Taiwan

来源：

PLANT CELL REPORTS | 1999年 / 19卷 / 02期

关键词：

Oncidium; somatic embryogenesis; thidiazuron;

D O I：

10.1007/s002990050724

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Segments taken from young leaves of an orchid (Oncidium Cower Ramsey) produced clusters of somatic embryos directly from epidermal and mesophyll cells of leaf tips and wound surfaces without an intervening callus within 1 month when cultured on a Gelrite(TM)-gelled 1/2-MS basal medium supplemented with a low dosage (0.3-1 mg/l) of thidiazuron. Subculturing of these embryo clusters produced more embryos and subsequent plantlet formation on the same medium. The high-frequency embryogenesis of these leaf cells in this orchid is strong evidence of their totipotency, and further modification of the protocol for plant formation could be useful for the mass propagation and transformation of selected elite lines.

引用

页码：143 / 149

页数：7

共 15 条

[1]

BEGUM AA, 1994, J JPN SOC HORTIC SCI, V63, P419, DOI 10.2503/jjshs.63.419

[2]

Plant regeneration from callus culture of Cymbidium ensifolium var. misericors [J].