Safety and efficacy of hepatitis B surface antigen-pulsed dendritic cells in human volunteers

被引：46

作者：

Akbar, SMF ^{[1
]}

Furukawa, S ^{[1
]}

Onji, M ^{[1
]}

Murata, Y ^{[1
]}

Niya, T ^{[1
]}

Kanno, S ^{[1
]}

Murakami, H ^{[1
]}

Horiike, N ^{[1
]}

机构：

[1] Ehime Univ, Sch Med, Dept Internal Med 3, Shigenobu, Ehime 790295, Japan

来源：

HEPATOLOGY RESEARCH | 2004年 / 29卷 / 03期

关键词：

hepatitis B surface antigen; dendritic cells; anti-HBs; human volunteer;

D O I：

10.1016/j.hepres.2004.03.003

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Hepatitis B surface antigen (HBsAg)-pulsed murine spleen dendritic cells (DCs) have shown tremendous therapeutic potentials in chronic hepatitis B virus (HBV) carriers however, there has been no study regarding the feasibility of using HBsAg-pulsed DCs in human. Five human healthy volunteers with no apparent concomitant diseases were enrolled in this study. DCs were enriched from peripheral blood of each volunteer in endotoxin-free and sterilized conditions. HBsAg-pulsed DCs were prepared by culturing DCs with a commercial-available human grade vaccine containing HBsAg. After assessing the expression of HLA DR and CD86 on HBsAg-pulsed DCs, 5 million HBsAg-pulsed DCs were injected intradermally, once, to each volunteer. The volunteers were serially observed for safety and efficacy of administration of HBsAg-pulsed DCs. No evidence of physical, biochemical, and immunological abnormalities were documented in any volunteer during the next 28 days following administration of HBsAg-pulsed DCs. A single administration of HBsAg-pulsed DCs resulted in upregulation of anti-HBs in two anti-HBs(+) volunteers. Moreover, anti-HBs were detected in two anti-HBs- volunteer, 2 weeks after administration of HBsAg-pulsed DCs. This study provides the scientific and ethical basis for using HBsAg-pulsed DCs for therapeutic and prophylactic purposes in patients with chronic hepatitis B and non-responders to hepatitis B vaccine, respectively. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：136 / 141

页数：6

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