Ultrasensitive ATP detection using firefly luciferase entrapped in sugar-modified sol-gel-derived silica

被引：98

作者：

Cruz-Aguado, JA

Chen, Y

Zhang, Z

Elowe, NH

Brook, MA

Brennan, JD

机构：

[1] McMaster Univ, Dept Chem, Hamilton, ON L8S 4M1, Canada

[2] McMaster Univ, Dept Biochem, HTS Lab, Hamilton, ON L8N 3Z5, Canada

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 22期

关键词：

D O I：

10.1021/ja0490424

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Firefly luciferase (FL) was entrapped in sol-gel-derived silica containing precursors based on covalent linkage of d-gluconolactone or d-maltonolactone to (aminopropyl)triethoxysilane to form N-(3-triethoxysilylpropyl)gluconamide or N-(3-triethoxysilylpropyl)maltonamide. The enzyme was active and stable in this material and showed catalytic constants close to those in solution. As little as 20 amol ATP could be detected with the entrapped FL, and the entrapped enzyme could be used over several cycles. Copyright © 2004 American Chemical Society.

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页码：6878 / 6879

页数：2

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