Arginase expression in mouse embryonic development

被引：18

作者：

Yu, H

Iyer, RK

Yoo, PK

Kern, RM

Grody, WW

Cederbaum, SD

机构：

[1] Univ Calif Los Angeles, Mental Retardat Res Ctr, Los Angeles, CA 90024 USA

[2] Univ Calif Los Angeles, Dept Pediat, Los Angeles, CA 90024 USA

[3] Univ Calif Los Angeles, Dept Pathol & Lab Med, Los Angeles, CA 90024 USA

[4] Univ Calif Los Angeles, Dept Pediat, Los Angeles, CA 90024 USA

来源：

MECHANISMS OF DEVELOPMENT | 2002年 / 115卷 / 1-2期

关键词：

arginase; embryonic development; ganglion cell; leukocyte; in situ hybridization; expression;

D O I：

10.1016/S0925-4773(02)00089-8

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We are using the model of the developing mouse embryo to elucidate the pattern of arginase expression in mammalian cells in normal animals and in arginase I (AI) deficiency during development by digoxigenin-labeled RNA in situ hybridization. Our goal is to understand the regulation of these isozymes, with the expectation that this knowledge will help patients suffering from AI deficiency. We found that AI mRNA was widely and strongly expressed in the normal developing mouse embryo; in contrast, a relatively strong All mRNA signal was found only in the intestine. In the AI knockout mouse embryo, no All overexpression was found. These results indicated that arginases are needed in mouse embryonic development and AI is the principal form required. The strong AI expression in the peripheral nervous system suggests that the pathogenesis of the neurological retardation in AI deficiency may be conditioned by AI deficiency in the nervous system during embryonic development. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：151 / 155

页数：5

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[2]

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[3]

FOZARD JR, 1980, SCIENCE, V208, P505

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