Structure, organization and expression of the eukaryotic translation initiation factor 5, eIF-5, gene in Zea mays

被引：3

作者：

Ribera, IL ^{[1
]}

Puigdomènech, P ^{[1
]}

机构：

[1] Inst Mol Biol Barcelona, Dept Mol Genet, CSIC, CID, Barcelona 08034, Spain

来源：

GENE | 1999年 / 240卷 / 02期

关键词：

eukaryotic translation initiation factors; eIF-5; gene; maize; meristem;

D O I：

10.1016/S0378-1119(99)00438-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The maize genomic DNA sequence encoding the eukaryotic translation initiation factor 5 (eIF-5) has been isolated from genomic library of maize seedlings and the exon-intron structure determined (accession number AJ132240). The length of genomic DNA sequenced was about 7 kb and contained two exons with the translation start site in exon 2. The only intron is located in the non-coding 5' region and it is 1298 bp long with the splice acceptor and donor sites conforming to the AG/GT rules. Repetitive sequence fragments are located in the 5' and 3' intergenic region. The accumulation of eIF-5 mRNA was studied by RNA blot and in situ hybridization. The observed distribution of mRNA may correlate with the function of the protein, as it appears to be highly abundant in tissues where the proportion of cells actively dividing is very high, such as meristematic regions. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：355 / 359

页数：5

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