Measurement of diffusion constants for nucleic acids by NMR

被引：108

作者：

Lapham, J ^{[1
]}

Rife, JP ^{[1
]}

Moore, PB ^{[1
]}

Crothers, DM ^{[1
]}

机构：

[1] YALE UNIV,DEPT CHEM,NEW HAVEN,CT 06520

来源：

JOURNAL OF BIOMOLECULAR NMR | 1997年 / 10卷 / 03期

关键词：

translational self-diffusion; spin echo; pulsed field-gradient; hairpin; duplex;

D O I：

10.1023/A:1018310702909

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pulsed field-gradient NMR experiments can be used to measure the diffusion constants of nucleic acids. The diffusion constants measured in this way for double-helical DNAs of defined length agree well both with theory and with measurements done using other techniques. When applied to RNAs, this experiment easily distinguishes duplex RNAs from RNA hairpins and thus it can solve one of the perennial problems faced by RNA spectroscopists, i.e. assessing whether their samples are monomeric or not.

引用

页码：255 / 262

页数：8

共 36 条

[1] DISTINGUISHING BETWEEN DUPLEX AND HAIRPIN FORMS OF RNA BY N-15-H-1 HETERONUCLEAR NMR [J].