Validation of Five-Colony Pool Analysis Using Multiplex Real-Time PCR for Detection of Diarrheagenic Escherichia coli

被引：35

作者：

Barletta, F. ^{[1
]}

Ochoa, T. J. ^{[1
,2
]}

Ecker, L. ^{[3
]}

Gil, A. I. ^{[3
]}

Lanata, C. F. ^{[3
]}

Cleary, T. G. ^{[2
]}

机构：

[1] Univ Peruana Cayetano Heredia, Inst Trop Med, Lima 33, Peru

[2] Univ Texas Houston, Sch Publ Hlth, Ctr Infect Dis, Houston, TX USA

[3] Inst Invest Nutr, Lima, Peru

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 06期

关键词：

CHILDREN;

D O I：

10.1128/JCM.00608-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.

引用

页码：1915 / 1917

页数：3

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