Combined MRI-PET dissects dynamic changes in plant structures and functions

被引：215

作者：

Jahnke, Siegfried ^{[1
,2
]}

Menzel, Marion I. ^{[1
]}

van Dusschoten, Dagmar ^{[1
]}

Roeb, Gerhard W. ^{[1
]}

Buehler, Jonas ^{[1
]}

Minwuyelet, Senay ^{[1
]}

Bluemler, Peter ^{[1
]}

Temperton, Vicky M. ^{[1
]}

Hombach, Thomas ^{[1
]}

Streun, Matthias ^{[3
]}

Beer, Simone ^{[3
]}

Khodaverdi, Maryam ^{[3
]}

Ziemons, Karl ^{[3
]}

Coenen, Heinz H. ^{[4
]}

Schurr, Ulrich ^{[1
]}

机构：

[1] Forschungszentrum, ICG Phytosphere 3, D-52425 Julich, Germany

[2] Univ Duisburg Essen, FB Biol & Geog, D-45117 Essen, Germany

[3] Forschungszentrum, ZEL Cent Inst Elect, D-52425 Julich, Germany

[4] Forschungszentrum, INB Nucl Chem 5, D-52425 Julich, Germany

来源：

PLANT JOURNAL | 2009年 / 59卷 / 04期

关键词：

carbon-11 (C-11); co-registration; functional imaging (3D); magnetic resonance imaging (MRI); non-invasive method; positron emission tomography (PET); SUGAR-BEET; MAGNETIC-RESONANCE; PHLOEM; SYSTEM; TRANSPORT; SOIL; TRANSLOCATION; CARBON; ROOTS; XYLEM;

D O I：

10.1111/j.1365-313X.2009.03888.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Unravelling the factors determining the allocation of carbon to various plant organs is one of the great challenges of modern plant biology. Studying allocation under close to natural conditions requires noninvasive methods, which are now becoming available for measuring plants on a par with those developed for humans. By combining magnetic resonance imaging (MRI) and positron emission tomography (PET), we investigated three contrasting root/shoot systems growing in sand or soil, with respect to their structures, transport routes and the translocation dynamics of recently fixed photoassimilates labelled with the short-lived radioactive carbon isotope C-11. Storage organs of sugar beet (Beta vulgaris) and radish plants (Raphanus sativus) were assessed using MRI, providing images of the internal structures of the organs with high spatial resolution, and while species-specific transport sectoralities, properties of assimilate allocation and unloading characteristics were measured using PET. Growth and carbon allocation within complex root systems were monitored in maize plants (Zea mays), and the results may be used to identify factors affecting root growth in natural substrates or in competition with roots of other plants. MRI-PET co-registration opens the door for non-invasive analysis of plant structures and transport processes that may change in response to genomic, developmental or environmental challenges. It is our aim to make the methods applicable for quantitative analyses of plant traits in phenotyping as well as in understanding the dynamics of key processes that are essential to plant performance.

引用

页码：634 / 644

页数：11

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