Sensitivity of native and cloned hippocampal delayed-rectifier potassium channels to verapamil

The effects of the phenylalkylamine verapamil on native and cloned hippocampal voltage-operated potassium channels were investigated. Native channels were studied in acutely isolated CAI neurons from the guinea pig with the whole-cell patch-clamp technique. Cloned channels were expressed in oocytes of Xenopus laevis and studied with the two-electrode voltage-clamp technique. Native potassium channels: Verapamil suppressed the potassium currents in micro- and submicromolar concentrations. The current suppression increased during the voltage step. The IC,, value of verapamil was 3 mu mol/l and the Hill coefficient was 0.5 indicating a mixed population of potassium channels with distinct verapamil sensitivity. Cloned potassium channels: The hippocampal potassium channels Kv1.1, Kv1.2, Kv1.3, Kv2.1, Kv3.1 and Kv3.2 were affected by verapamil in micromolar concentrations. The effect increased with depolarization time, was voltage-dependent, reached 90% of the maximum within around 40 s after start of verapamil application, recovered slowly after wash-out and did not reach control values even after wash-out times of six minutes. The IC50 values differed markedly and were 35 mu mol/l for the Kv1.1 channel, 98 mu mol/l for the Kv1.2 channel, 12 mu mol/l for the Kv1.3 channel, 226 mu mol/l for the Kv2.1 channel, 6 mu mol/l for the Kv3.1 channel and 11 mu mol/l for the Kv3.2 channel. (C) 2000 Elsevier Science Ltd. All rights reserved.