Caspase 9 activation by the dsRNA-dependent protein kinase, PKR: molecular mechanism and relevance

被引：49

作者：

Gil, J ^{[1
]}

Garcia, MA ^{[1
]}

Esteban, M ^{[1
]}

机构：

[1] CSIC, Ctr Nacl Biotecnol, Dept Mol & Cellular Biol, Madrid 28049, Spain

来源：

FEBS LETTERS | 2002年 / 529卷 / 2-3期

关键词：

apoptosis; double-stranded RNA-dependent protein kinase; caspase; 9; cytochrome c; mitochondria;

D O I：

10.1016/S0014-5793(02)03348-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The double-stranded RNA-dependent protein kinase (PKR) induces apoptosis by activation of the FADD/caspase 8 pathway. Here we show that upon PKR expression, caspase 9 is processed and activated, correlating with the translocation of cytochrome c to the cytoplasm and breakdown of mitochondrial potential upon Bax insertion. However, treatment of cells with an inhibitor of caspase 9 could not prevent PKR-induced apoptosis. During PKR-induced apoptosis, caspase 9 is activated downstream of caspase 8. Our findings revealed that caspase 9, although dispensable, is a mediator of PKR-induced cell death. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：249 / 255

页数：7

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