3D characterization of morphological changes in the intervertebral disc and endplate during aging: A propagation phase contrast synchrotron micro-tomography study

被引：33

作者：

Cao, Yong ^{[1
]}

Liao, Shenghui ^{[2
]}

Zeng, Hao ^{[1
]}

Ni, Shuangfei ^{[1
]}

Tintani, Francis ^{[3
]}

Hao, Yongqiang ^{[4
]}

Wang, Lei ^{[5
]}

Wu, Tianding ^{[1
]}

Lu, Hongbin ^{[6
]}

Duan, Chunyue ^{[1
]}

Hu, Jianzhong ^{[1
]}

机构：

[1] Cent South Univ, Xiangya Hosp, Dept Spine Surg, Changsha 410008, Hunan, Peoples R China

[2] Cent South Univ, Sch Informat Sci & Engn, Changsha 410008, Hunan, Peoples R China

[3] Johns Hopkins Univ, Pediat Endocrinol, Baltimore, MD 21203 USA

[4] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Dept Orthopaed, Shanghai, Peoples R China

[5] Southern Med Univ, Nanfang Hosp, Dept Orthopaed & Traumatol, Guangzhou 51000, Guangdong, Peoples R China

[6] Cent South Univ, Xiangya Hosp, Res Ctr Sports Med, Dept Sports Med, Changsha 410008, Hunan, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

AGE-RELATED-CHANGES; LOW-BACK; X-RAY; COMPUTED-TOMOGRAPHY; DEGENERATION; PREVALENCE; SPINE; PATHOPHYSIOLOGY; VASCULARIZATION; ASSOCIATION;

D O I：

10.1038/srep43094

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

A better understanding of functional changes in the intervertebral disc (IVD) and interaction with endplate is essential to elucidate the pathogenesis of IVD degeneration disease (IDDD). To date, the simultaneous depiction of 3D micro-architectural changes of endplate with aging and interaction with IVD remains a technical challenge. We aim to characterize the 3D morphology changes of endplate and IVD during aging using PPCST. The lumbar vertebral level 4/5 IVDs harvested from 15-day-, 4-and 24-month-old mice were initially evaluated by PPCST with histological sections subsequently analyzed to confirm the imaging efficiency. Quantitative assessments of age-related trends after aging, including mean diameter, volume fraction and connectivity of the canals, and endplate porosity and thickness, reached a peak at 4 months and significantly decreased at 24 months. The IVD volume consistently exhibited same trend of variation with the endplate after aging. In this study, PPCST simultaneously provided comprehensive details of 3D morphological changes of the IVD and canal network in the endplate and the interaction after aging. The results suggest that PPCST has the potential to provide a new platform for attaining a deeper insight into the pathogenesis of IDDD, providing potential therapeutic targets.

引用

页数：12

共 52 条

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