RETRACTED: TAF1 activates transcription by phosphorylation of serine 33 in histone H2B (Retracted Article)

被引：50

作者：

Maile, T

Kwoczynski, S

Katzenberger, RJ

Wassarman, DA

Sauer, F

机构：

[1] Univ Wisconsin, Dept Pharmacol, Madison, WI 53706 USA

[2] Univ Calif Riverside, Dept Biochem, Riverside, CA 95121 USA

[3] Heidelberg Univ, Zentrum Mol Biol, D-69120 Heidelberg, Germany

来源：

SCIENCE | 2004年 / 304卷 / 5673期

关键词：

D O I：

10.1126/science.1095001

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dynamic changes in chromatin structure, induced by posttranslational modification of histones, play a fundamental role in regulating eukaryotic transcription. Here we report that histone H2B is phosphorylated at evolutionarily conserved Ser(33) (H2B-S33) by the carboxyl-terminal kinase domain (CTK) of the Drosophila TFIID subunit TAF1. Phosphorylation of H2B-S33 at the promoter of the cell cycle regulatory gene string and the segmentation gene giant coincides with transcriptional activation. Elimination of TAF1 CTK activity in Drosophila cells and embryos reduces transcriptional activation and phosphorylation of H2B-S33. These data reveal that H2B-S33 is a physiological substrate for the TAF1 CTK and that H2B-S33 phosphorylation is essential for transcriptional activation events that promote cell cycle progression and development.

引用

页码：1010 / 1014

页数：5

共 28 条

[1] Yeast TAF(II)90 is required for cell-cycle progression through G(2)/M but not for general transcription activation [J].