Crystallization and preliminary crystallographic analysis of a novel plant type III polyketide synthase that produces pentaketide chromone

被引：7

作者：

Morita, Hiroyuki

Kondo, Shin

Abe, Tsuyoshi

Noguchi, Hiroshi

Sugio, Shigetoshi

Abe, Ikuro

Kohno, Toshiyuki

机构：

[1] Mitsubishi Kagaku Inst Life Sci, Tokyo 1948511, Japan

[2] ZOEGENE Corp, Kanagawa 2278502, Japan

[3] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka 4228526, Japan

[4] Univ Shizuoka, COE21 Program, Shizuoka 4228526, Japan

[5] Japan Sci & Technol Agcy, PRESTO, Kawagoe, Saitama 3320012, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2006年 / 62卷

关键词：

D O I：

10.1107/S174430910602968X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pentaketide chromone synthase (PCS) from Aloe arborescens is a novel plant-specific type III polyketide synthase that catalyzes the formation of 5,7-dihydroxy-2-methylchromone from five molecules of malonyl-CoA. Recombinant PCS expressed in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group P2(1), with unit-cell parameters a = 73.2, b = 88.4, c = 70.0 angstrom, gamma = 90.0, beta = 95.6 degrees. Diffraction data were collected to 1.6 angstrom resolution using synchrotron radiation at BL24XU of SPring-8.

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收藏

页码：899 / 901

页数：3

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