Resveratrol protects cardiomyocytes from doxorubicin-induced apoptosis through the AMPK/P53 pathway

被引：64

作者：

Liu, Mi-Hua ^{[1
]}

Lin, Xiao-Long ^{[2
]}

Guo, Dong-Ming ^{[3
]}

Zhang, Yuan ^{[4
]}

Yuan, Cong ^{[5
]}

Tan, Tian-Ping ^{[1
]}

Chen, Yu-Dan ^{[1
]}

Wu, Shao-Jian ^{[1
]}

Ye, Zu-Feng ^{[1
]}

He, Jun ^{[1
]}

机构：

[1] Univ South China, Affiliated Nanhua Hosp, Dept Clin Lab, 336 Dongfeng South Rd, Hengyang 421001, Hunan, Peoples R China

[2] Guangzhou Med Univ, Peoples Hosp Huizhou 3, Dept Pathol, Huizhou 516002, Guangdong, Peoples R China

[3] Univ South China, Lab Clin Anat, Hengyang 421001, Hunan, Peoples R China

[4] Mawangdui Hosp, Dept Pathol, Changsha 410016, Hunan, Peoples R China

[5] First Hosp Changsha, Dept Cardiol, Changsha 410005, Hunan, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2016年 / 13卷 / 02期

关键词：

resveratrol; doxorubicin; apoptosis; P53; adenosine monophosphate-activated protein kinase; INDUCED CARDIOTOXICITY; CELL-DEATH; PC12; CELLS; KINASE; INHIBITION; ACTIVATION; STRESS; ALPHA; P53;

D O I：

10.3892/mmr.2015.4665

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Doxorubicin (DOX) is an efficient drug used in cancer therapy; however, it has severe cardiotoxic side effects. The aim of the present study was to investigate the effects of resveratrol on the adenosine monophosphate-activated protein kinase (AMPK)/P53 pathway in mediating DOX-induced cytotoxicity. H9c2 cells were exposed to 5 mu M DOX for 24 h to establish a model of DOX-induced cardiotoxicity. DOX administration amplified P53 and B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax) expression and decreased Bcl-2 expression in H9c2 cells. Resveratrol increased the cell viability and decreased the apoptotic rate. In addition, resveratrol markedly increased the phosphorylation of AMPK. Of note, resveratrol protected against DOX-induced increases of P53 and Bax and also prevented the downregulation of Bcl-2 in H9c2 cells. Furthermore, AMPK inhibitor Compound C abolished the protective effects of resveratrol. The results of the present study therefore indicated that resveratrol protected H9c2 cells from DOX-induced apoptosis via the AMPK/P53 pathway.

引用

页码：1281 / 1286

页数：6

共 25 条

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Hydrogen peroxide inhibits mTOR signaling by activation of AMPKα leading to apoptosis of neuronal cells [J].