Isolation and characterization of the Spindly homologue from tomato

被引:9
作者
Greb, T [1 ]
Schmitz, G [1 ]
Theres, K [1 ]
机构
[1] Max Planck Inst Plant Breeding Res, D-50829 Cologne, Germany
关键词
GA signalling; gibberellins; Procera; Spindly;
D O I
10.1093/jxb/erf033
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The SPINDLY (SPY) gene is a crucial component of the gibberellin signal transduction pathway in Arabidopsis thaliana (L.) Heynh. In this study, the cloning of the SPINDLY-orthologous gene (LeSpy) from tomato (Lycopersicon esculentum Mill.) and its characterization are reported. SPY and LeSpy show high sequence similarity along their entire lengths, which is reflected in the conservation of exon-intron structure and of all sequence motives previously identified. To analyse the relationship between the Arabidopsis spindly and the tomato procera mutant, which show similar phenotypes, the LeSpy gene was characterized in wild-type and procera tomato plants. These analyses as well as mapping of LeSpy revealed that LeSpy and Procera are different genes.
引用
收藏
页码:1829 / 1830
页数:2
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