Postadhesive technique for archival paraffin sections

被引:3
作者
Pateraki, M [1 ]
Kontogeorgos, G [1 ]
机构
[1] GEN HOSP ATHENS,DEPT PATHOL,ATHENS 11527,GREECE
关键词
adhesive materials; antigen unmasking; archival material; enzymatic treatment; immunohistochemistry; microwaves;
D O I
10.3109/10520299709082232
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We present a postadhesive protocol for adhering paraffin sections of archival material to microscope slides, Appropriately posttreated sections, subsequently processed for immunohistochemistry, remained attached to the slides and were well preserved with no signs of artifacts, such as scratching and shrinkage, The immuno-histochemical staining was intense and antigenspecific without nonspecific background, Specific staining intensity was equal to that produced in untreated control sections; however, the latter became partially or fully detached from the slides, The postadhesion protocol may be used with modern techniques and is recommended for reclaiming use of otherwise unsuitable paraffin sections of archival material.
引用
收藏
页码:168 / 170
页数:3
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