Essential requirement for two-pore channel 1 in NAADP-mediated calcium signaling

被引：334

作者：

Brailoiu, Eugen ^{[2
]}

Churamani, Dev ^{[1
]}

Cai, Xinjiang ^{[3
]}

Schrlau, Michael G. ^{[4
]}

Brailoiu, G. Cristina ^{[2
]}

Gao, Xin ^{[2
]}

Hooper, Robert ^{[1
]}

Boulware, Michael J. ^{[5
]}

Dun, Nae J. ^{[2
]}

Marchant, Jonathan S. ^{[5
]}

Patel, Sandip ^{[1
]}

机构：

[1] UCL, Dept Cell & Dev Biol, London WC1E 6BT, England

[2] Temple Univ, Dept Pharmacol, Sch Med, Philadelphia, PA 19140 USA

[3] Duke Univ, Div Cardiol, Dept Med, Med Ctr, Durham, NC 27710 USA

[4] Univ Penn, Dept Mech Engn & Appl Mech, Philadelphia, PA 19104 USA

[5] Univ Minnesota, Dept Pharmacol, Sch Med, Minneapolis, MN 55455 USA

来源：

JOURNAL OF CELL BIOLOGY | 2009年 / 186卷 / 02期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会; 美国国家卫生研究院;

关键词：

ADENINE-DINUCLEOTIDE PHOSPHATE; CYCLIC ADP-RIBOSE; INOSITOL TRISPHOSPHATE; MOBILIZES CALCIUM; CA2+ RELEASE; MOLECULAR EVOLUTION; PANCREATIC ACINAR; SKELETAL-MUSCLE; RECEPTOR; PROTEIN;

D O I：

10.1083/jcb.200904073

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nicotinic acid adenine dinucleotide phosphate (NAADP) is a widespread and potent calcium-mobilizing messenger that is highly unusual in activating calcium channels located on acidic stores. However, the molecular identity of the target protein is unclear. In this study, we show that the previously uncharacterized human two-pore channels (TPC1 and TPC2) are endolysosomal proteins, that NAADP-mediated calcium signals are enhanced by overexpression of TPC1 and attenuated after knockdown of TPC1, and that mutation of a single highly conserved residue within a putative pore region abrogated calcium release by NAADP. Thus, TPC1 is critical for NAADP action and is likely the long sought after target channel for NAADP.

引用

页码：201 / 209

页数：9

共 44 条

[1] Calcium signaling by cyclic ADP-ribose, NAADP, and inositol trisphosphate are involved in distinct functions in ascidian oocytes [J].

Albrieux, M ;

Lee, HC ;

Villaz, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (23) :14566-14574

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[3] Nicotinic acid adenine dinucleotide phosphate and cyclic ADP-ribose regulate TRPM2 channels in T lymphocytes [J].

Beck, Andreas ;

Kolisek, Martin ;

Bagley, Leigh Anne ;

Fleig, Andrea ;

Penner, Reinhold .

FASEB JOURNAL, 2006, 20 (07) :962-+

[4] Solubilization of receptors for the novel Ca2+-mobilizing messenger, nicotinic acid adenine dinucleotide phosphate [J].

Berridge, G ;

Dickinson, G ;

Parrington, J ;

Galione, A ;

Patel, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (46) :43717-43723

[5] The versatility and universality of calcium signalling [J].

Berridge, MJ ;

Lipp, P ;

Bootman, MD .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2000, 1 (01) :11-21

[6] Nicotinic acid adenine dinucleotide phosphate potentiates neurite outgrowth [J].

Brailoiu, E ;

Hoard, JL ;

Filipeanu, CM ;

Brailoiu, GC ;

Dun, SL ;

Patel, S ;

Dun, NJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (07) :5646-5650

[7] Messenger-specific role for nicotinic acid adenine dinucleotide phosphate in neuronal differentiation [J].

Brailoiu, Eugen ;

Churamani, Dev ;

Pandey, Vinita ;

Brailoiu, G. Cristina ;

Tuluc, Florin ;

Patel, Sandip ;

Dun, Nae J. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (23) :15923-15928

[8] Molecular Evolution and Functional Divergence of the Ca2+ Sensor Protein in Store-operated Ca2+ Entry: Stromal Interaction Molecule [J].

Cai, Xinjiang .

PLOS ONE, 2007, 2 (07)

[9] Molecular evolution of the ankyrin gene family [J].

Cai, XJ ;

Zhang, YH .

MOLECULAR BIOLOGY AND EVOLUTION, 2006, 23 (03) :550-558

[10] NAADP mobilizes calcium from acidic organelles through two-pore channels [J].

Calcraft, Peter J. ;

Ruas, Margarida ;

Pan, Zui ;

Cheng, Xiaotong ;

Arredouani, Abdelilah ;

Hao, Xuemei ;

Tang, Jisen ;

Rietdorf, Katja ;

Teboul, Lydia ;

Chuang, Kai-Ting ;

Lin, Peihui ;

Xiao, Rui ;

Wang, Chunbo ;

Zhu, Yingmin ;

Lin, Yakang ;

Wyatt, Christopher N. ;

Parrington, John ;

Ma, Jianjie ;

Evans, A. Mark ;

Galione, Antony ;

Zhu, Michael X. .

NATURE, 2009, 459 (7246) :596-U130

← 1 2 3 4 5 →