Large-scale analysis of glycoproteins by LC-MS method

被引：3

作者：

Kaji, Hiroyuki ^{[1
]}

Isobe, Toshiaki ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Grad Sch Sci & Engn, Dept Chem, Tokyo 1920397, Japan

来源：

TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY | 2006年 / 18卷 / 103期

关键词：

proteomics; glycoprotein; mass spectrometry; lectin; LC-MS;

D O I：

10.4052/tigg.18.313

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review presents a strategy and applications of the large-scale identification of N-glycosylated proteins and their glycosylated sites. The technology is based on the affinity capture of glycopeptides with lectins, the enzyme-mediated stable isotope-labeling of N-glycosylated sites, and the liquid chromatography/mass spectrometry (LC-MS) shotgun analysis.

引用

页码：313 / 322

页数：10

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