TMEM150C/Tentonin3 Is a Regulator of Mechano-gated Ion Channels

被引:66
作者
Anderson, Evan O. [1 ]
Schneider, Eve R. [1 ]
Matson, Jon D. [1 ]
Gracheva, Elena O. [1 ,2 ,3 ]
Bagriantsev, Sviatoslav N. [1 ]
机构
[1] Yale Univ, Sch Med, Dept Cellular & Mol Physiol, 333 Cedar St, New Haven, CT 06520 USA
[2] Yale Univ, Sch Med, Dept Neurosci, 333 Cedar St, New Haven, CT 06520 USA
[3] Yale Univ, Sch Med, Program Cellular Neurosci Neurodegenerat & Repair, 333 Cedar St, New Haven, CT 06520 USA
基金
美国国家科学基金会;
关键词
PIEZO1; MECHANOSENSITIVITY; MEMBRANE; TREK-1; TRAAK; TOUCH; MECHANOTRANSDUCTION; ACTIVATION; MUTATIONS; PATCH;
D O I
10.1016/j.celrep.2018.03.094
中图分类号
Q2 [细胞生物学];
学科分类号
071013 [干细胞生物学];
摘要
Neuronal mechano-sensitivity relies on mechano-gated ion channels, but pathways regulating their activity remain poorly understood. TMEM150C was proposed to mediate mechano-activated current in proprioceptive neurons. Here, we studied functional interaction of TMEM150C with mechano-gated ion channels from different classes (Piezo2, Piezo1, and the potassium channel TREK-1) using two independent methods of mechanical stimulation. We found that TMEM150C significantly prolongs the duration of the mechano-current produced by all three channels, decreases apparent activation threshold in Piezo2, and induces persistent current in Piezo1. We also show that TMEM150C is co-expressed with Piezo2 in trigeminal neurons, expanding its role beyond proprioceptors. Finally, we cloned TMEM150C from the trigeminal neurons of the tactile-foraging domestic duck and showed that it functions similarly to the mouse ortholog, demonstrating evolutionary conservation among vertebrates. Our studies reveal TMEM150C as a general regulator of mechano-gated ion channels from different classes.
引用
收藏
页码:701 / 708
页数:8
相关论文
共 51 条
[1]
Dehydrated hereditary stomatocytosis linked to gain-of-function mutations in mechanically activated PIEZO1 ion channels [J].
Albuisson, Juliette ;
Murthy, Swetha E. ;
Bandell, Michael ;
Coste, Bertrand ;
Louis-dit-Picard, Helene ;
Mathur, Jayanti ;
Feneant-Thibault, Madeleine ;
Tertian, Gerard ;
de Jaureguiberry, Jean-Pierre ;
Syfuss, Pierre-Yves ;
Cahalan, Stuart ;
Garcon, Loic ;
Toutain, Fabienne ;
Rohrlich, Pierre Simon ;
Delaunay, Jean ;
Picard, Veronique ;
Jeunemaitre, Xavier ;
Patapoutian, Ardem .
NATURE COMMUNICATIONS, 2013, 4
[2]
TREK-1, a K+ channel involved in polymodal pain perception [J].
Alloui, Abdelkrim ;
Zimmermann, Katharina ;
Mamet, Julien ;
Duprat, Fabrice ;
Noel, Jacques ;
Chemin, Jean ;
Guy, Nicolas ;
Blondeau, Nicolas ;
Voilley, Nicolas ;
Rubat-Coudert, Catherine ;
Borsotto, Marc ;
Romey, Georges ;
Heurteaux, Catherine ;
Reeh, Peter ;
Eschalier, Alain ;
Lazdunski, Michel .
EMBO JOURNAL, 2006, 25 (11) :2368-2376
[3]
Xerocytosis is caused by mutations that alter the kinetics of the mechanosensitive channel PIEZO1 [J].
Bae, Chilman ;
Gnanasambandam, Radhakrishnan ;
Nicolai, Chris ;
Sachs, Frederick ;
Gottlieb, Philip A. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (12) :E1162-E1168
[4]
Metabolic and thermal stimuli control K2P2.1 (TREK-1) through modular sensory and gating domains [J].
Bagriantsev, Sviatoslav N. ;
Clark, Kimberly A. ;
Minor, Daniel L., Jr. .
EMBO JOURNAL, 2012, 31 (15) :3297-3308
[5]
Multiple modalities converge on a common gate to control K2P channel function [J].
Bagriantsev, Sviatoslav N. ;
Peyronnet, Remi ;
Clark, Kimberly A. ;
Honore, Eric ;
Minor, Daniel L., Jr. .
EMBO JOURNAL, 2011, 30 (17) :3594-3606
[6]
High-speed pressure clamp [J].
Besch, SR ;
Suchyna, T ;
Sachs, F .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2002, 445 (01) :161-166
[7]
Regulation of Piezo Channels by Cellular Signaling Pathways [J].
Borbiro, I. ;
Rohacs, T. .
PIEZO CHANNELS, 2017, 79 :245-261
[8]
Activation of TRPV1 channels inhibits mechanosensitive Piezo channel activity by depleting membrane phosphoinositides [J].
Borbiro, Istvan ;
Badheka, Doreen ;
Rohacs, Tibor .
SCIENCE SIGNALING, 2015, 8 (363)
[9]
Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K+ channels [J].
Brohawn, Stephen G. ;
Su, Zhenwei ;
MacKinnon, Roderick .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (09) :3614-3619
[10]
Crystal Structure of the Human K2P TRAAK, a Lipid- and Mechano-Sensitive K+ Ion Channel [J].
Brohawn, Stephen G. ;
del Marmol, Josefina ;
MacKinnon, Roderick .
SCIENCE, 2012, 335 (6067) :436-441