Targeted Peptide Measurements in Biology and Medicine: Best Practices for Mass Spectrometry- based Assay Development Using a Fit- for- Purpose Approach

被引：429

作者：

Carr, Steven A. ^{[1
]}

Abbatiello, Susan E. ^{[1
]}

Ackermann, Bradley L. ^{[2
]}

Borchers, Christoph ^{[3
]}

Domon, Bruno ^{[4
]}

Deutsch, Eric W. ^{[5
]}

Grant, Russell P. ^{[6
]}

Hoofnagle, Andrew N. ^{[7
]}

Huettenhain, Ruth ^{[8
,9
]}

Koomen, John M. ^{[10
]}

Liebler, Daniel C. ^{[11
]}

Liu, Tao ^{[12
]}

MacLean, Brendan ^{[7
]}

Mani, D. R. ^{[1
]}

Mansfield, Elizabeth ^{[13
]}

Neubert, Hendrik ^{[14
]}

Paulovich, Amanda G. ^{[15
]}

Reiter, Lukas ^{[16
]}

Vitek, Olga ^{[17
]}

Aebersold, Ruedi ^{[8
]}

Anderson, Leigh ^{[18
]}

Bethem, Robert ^{[19
]}

Blonder, Josip ^{[20
]}

Boja, Emily ^{[20
]}

Botelho, Julianne ^{[21
]}

Boyne, Michael ^{[13
]}

Bradshaw, Ralph A. ^{[9
]}

Burlingame, Alma L. ^{[9
]}

Chan, Daniel ^{[22
]}

Keshishian, Hasmik ^{[1
]}

Kuhn, Eric ^{[1
]}

Kinsinger, Christopher ^{[20
]}

Lee, Jerry S. H. ^{[20
,22
]}

Lee, Sang-Won ^{[23
]}

Moritz, Robert ^{[5
]}

Oses-Prieto, Juan ^{[9
]}

Rifai, Nader ^{[24
]}

Ritchie, James ^{[25
]}

Rodriguez, Henry ^{[20
]}

Srinivas, Pothur R. ^{[26
]}

Townsend, R. Reid ^{[27
]}

Van Eyk, Jennifer ^{[22
]}

Whiteley, Gordon ^{[28
]}

Wiita, Arun ^{[9
]}

Weintraub, Susan ^{[29
]}

机构：

[1] Broad Inst MIT & Harvard, Cambridge, MA USA

[2] Eli Lilly & Co, Indianapolis, IN 46285 USA

[3] Univ Victoria, Victoria, BC, Canada

[4] Luxembourg Clin Prote Ctr, Luxembourg, Luxembourg

[5] Inst Syst Biol, Seattle, WA USA

[6] Lab Corp Amer, Burlington, NC USA

[7] Univ Washington, Seattle, WA 98195 USA

[8] Swiss Fed Inst Technol, Inst Mol Syst Biol, Zurich, Switzerland

[9] Univ Calif San Francisco, San Francisco, CA 94143 USA

[10] Univ S Florida, H Lee Moffitt Canc Ctr, Tampa, FL 33682 USA

[11] Vanderbilt Univ, Nashville, TN 37235 USA

[12] Pacific NW Natl Lab, Richland, WA 99352 USA

[13] US FDA, Silver Spring, MD USA

[14] Pfizer, Andover, MA USA

[15] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA

[16] Biognosys Schlieren, Zurich, Switzerland

[17] Purdue Univ, Purdue, IN USA

[18] SISCAPA Assay Technol Inc, Washington, DC USA

[19] RAB Consulting, Novato, CA USA

[20] NCI, NIH Bethesda, Bethesda, MD 20892 USA

[21] Ctr Dis Control & Prevent, Atlanta, GA USA

[22] Johns Hopkins Univ, Baltimore, MD USA

[23] Korea Univ, Seoul, South Korea

[24] Childrens Hosp, Boston, MA 02115 USA

[25] Emory Univ, Atlanta, GA 30322 USA

[26] NHLBI, NIH Bethesda, Bethesda, MD USA

[27] Washington Univ, St Louis, MO USA

[28] Liedos Biomed Res Inc, Frederick Natl Lab Canc Res, Washington, DC USA

[29] Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX 78229 USA

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2014年 / 13卷 / 03期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

PROTEIN IDENTIFICATION DATA; LC-MS/MS ASSAY; INBORN-ERRORS; ABSOLUTE QUANTIFICATION; QUANTITATIVE-ANALYSIS; BIOMARKER DISCOVERY; CLINICAL VALIDATION; PLASMA; VERIFICATION; BIOANALYSIS;

D O I：

10.1074/mcp.M113.036095

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Adoption of targeted mass spectrometry (MS) approaches such as multiple reaction monitoring (MRM) to study biological and biomedical questions is well underway in the proteomics community. Successful application depends on the ability to generate reliable assays that uniquely and confidently identify target peptides in a sample. Unfortunately, there is a wide range of criteria being applied to say that an assay has been successfully developed. There is no consensus on what criteria are acceptable and little understanding of the impact of variable criteria on the quality of the results generated. Publications describing targeted MS assays for peptides frequently do not contain sufficient information for readers to establish confidence that the tests work as intended or to be able to apply the tests described in their own labs. Guidance must be developed so that targeted MS assays with established performance can be made widely distributed and applied by many labs worldwide. To begin to address the problems and their solutions, a workshop was held at the National Institutes of Health with representatives from the multiple communities developing and employing targeted MS assays. Participants discussed the analytical goals of their experiments and the experimental evidence needed to establish that the assays they develop work as intended and are achieving the required levels of performance. Using this fit-for-purpose approach, the group defined three tiers of assays distinguished by their performance and extent of analytical characterization. Computational and statistical tools useful for the analysis of targeted MS results were described. Participants also detailed the information that authors need to provide in their manuscripts to enable reviewers and readers to clearly understand what procedures were performed and to evaluate the reliability of the peptide or protein quantification measurements reported. This paper presents a summary of the meeting and recommendations.

引用

页码：907 / 917

页数：11

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